FlyBase Allele Report: Dmel\Rac2[GD17536]

General Information

Symbol

Dmel\Rac2^GD17536

Species

D. melanogaster

Name

FlyBase ID

FBal0208222

Feature type

allele

Associated gene

Dmel\Rac2

Associated Insertion(s)

Carried in Construct

P{GD17536}

Also Known As

Rac2 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD17536}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD17536

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^Hml.Δ

(Okuda et al., 2016)

abnormal immune response | larval stage, with Scer\GAL4^Hml.PG

(Howell et al., 2012)

short lived | conditional, with Scer\GAL4^Hml.Δ

(Okuda et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr.PU

(Szuplewski et al., 2012)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

anterior dorsocentral bristle, with Scer\GAL4^pnr.PU

(Szuplewski et al., 2012)

chaeta, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

dorsal appendage, with Scer\GAL4^CY2

(Peters et al., 2013)

dorsal appendage, with Scer\GAL4^Vm26Aa.F

(Peters et al., 2013)

microchaeta, with Scer\GAL4^pnr.PU

(Szuplewski et al., 2012)

Detailed Description

Statement

Reference

Expression of Rac2^GD17536 under the control of Scer\GAL4^Hml.Δ results in significantly reduced survival when infected with Leishmania amastigotes, as compared to wild type.

(Okuda et al., 2016)

Expression of Rac2^GD17536 under the control of Scer\GAL4^Vm26Aa.F results in moderate dorsal appendage defects in 56% of cases and strong defects in 8% of cases, with the appendages being rough.

Expression of Rac2^GD17536 under the control of Scer\GAL4^CY2 results in moderate dorsal appendage defects in 36% of cases and strong defects in 11% of cases, with the appendages having a wide base.

(Peters et al., 2013)

Larvae expressing Rac2^GD17536 under the control of Scer\GAL4^Hml.PG show strongly reduced encapsulation (1%) of eggs when parasitised by the avirulent L. boulardi wasp strain G486. Plasmatocytes fully cover the egg, while lamellocytes partially cover it.

(Howell et al., 2012)

Expression of Rac2^GD17536 under the control of Scer\GAL4^pnr.PU results in loss of microchaetae and an absence of dorso-anterior macrochaetae in the central part of the notum.

(Szuplewski et al., 2012)

Adults expressing Rac2^GD17536 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 90-100% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

17536

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Rac2^GD17536, Scer\GAL4^pnr.PU has visible phenotype, non-suppressible by Cul4[+]/Cul4^G1-3

(Szuplewski et al., 2012)

Rac2^GD17536, Scer\GAL4^pnr.PU has visible phenotype, non-suppressible by l(2)dtl^c02261/l(2)dtl[+]

(Szuplewski et al., 2012)

Rac2^GD17536, Scer\GAL4^pnr.PU has visible phenotype, non-suppressible by l(2)dtl^NIG.11295R, Scer\GAL4^pnr.PU

(Szuplewski et al., 2012)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Cg.PA, Mtl^KK110274/Rac2^GD17536 is a non-enhancer of increased cell number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Rac2^GD17536, Scer\GAL4^Cg.PA is a non-enhancer of increased cell number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

NOT Suppressor of

Statement

Reference

Rac2^GD17536, Scer\GAL4^Cg.PA is a non-suppressor of increased cell number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Scer\GAL4^Cg.PA, Mtl^KK110274/Rac2^GD17536 is a non-suppressor of increased cell number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rac2^GD17536, Scer\GAL4^Vm26Aa.F has dorsal appendage phenotype, enhanceable by ttk[+]/ttk^twp

(Peters et al., 2013)

Rac2^GD17536, Scer\GAL4^CY2 has dorsal appendage phenotype, enhanceable by ttk[+]/ttk^twp

(Peters et al., 2013)

NOT suppressed by

Statement

Reference

Rac2^GD17536, Scer\GAL4^pnr.PU has microchaeta phenotype, non-suppressible by Cul4[+]/Cul4^G1-3

(Szuplewski et al., 2012)

Rac2^GD17536, Scer\GAL4^pnr.PU has anterior dorsocentral bristle phenotype, non-suppressible by Cul4[+]/Cul4^G1-3

(Szuplewski et al., 2012)

Rac2^GD17536, Scer\GAL4^pnr.PU has microchaeta phenotype, non-suppressible by l(2)dtl^c02261/l(2)dtl[+]

(Szuplewski et al., 2012)

Rac2^GD17536, Scer\GAL4^pnr.PU has anterior dorsocentral bristle phenotype, non-suppressible by l(2)dtl^c02261/l(2)dtl[+]

(Szuplewski et al., 2012)

Rac2^GD17536, Scer\GAL4^pnr.PU has microchaeta phenotype, non-suppressible by l(2)dtl^NIG.11295R, Scer\GAL4^pnr.PU

(Szuplewski et al., 2012)

Rac2^GD17536, Scer\GAL4^pnr.PU has anterior dorsocentral bristle phenotype, non-suppressible by l(2)dtl^NIG.11295R, Scer\GAL4^pnr.PU

(Szuplewski et al., 2012)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Cg.PA, Mtl^KK110274/Rac2^GD17536 is a non-enhancer of embryonic/larval hemocyte | third instar larval stage | increased number phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Rac2^GD17536, Scer\GAL4^Cg.PA is a non-enhancer of embryonic/larval hemocyte | third instar larval stage | increased number phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

NOT Suppressor of

Statement

Reference

Rac2^GD17536, Scer\GAL4^Cg.PA is a non-suppressor of embryonic/larval hemocyte | increased number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Scer\GAL4^Cg.PA, Mtl^KK110274/Rac2^GD17536 is a non-suppressor of embryonic/larval hemocyte | increased number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the dorsal appendage defects caused by expression of Rac2^GD17536 under the control of either Scer\GAL4^Vm26Aa.F or Scer\GAL4^CY2 is enhanced by ttk^twp/+.

(Peters et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v50349