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General Information
Symbol
Dmel\Rae1GD14705
Species
D. melanogaster
Name
FlyBase ID
FBal0208230
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Rae1GD14705 under the control of Scer\GAL4bam.T:Hsim\VP16 (in the presence of Dcr-2Scer\UAS.cDa to enhance RNAi) results in male sterility. Primary spermatocytes contain nuclear herniations, onion stage spermatids have nuclei and Nebenkern of varying sizes and spermatid bundles contain round, scattered and irregularly sized nuclei. Meiotic defects are seen, including irregular metaphase plates in metaphase I and lagging chromosomes and unbalanced chromosome segregation in anaphase I.

Brain neuroblasts of third instar larvae expressing Rae1GD14705 under the control of Scer\GAL4wor.PA have a significantly higher mitotic index compared to wild type. 70% of anaphase figures have defects, including lagging chromosomes, chromatin bridges and uneven chromosome segregation.

Adults expressing Rae1GD14705 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 0% or 60-70% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 30-40% or 90-100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Rae1GD14705
Name Synonyms
Secondary FlyBase IDs
    References (6)