FlyBase Allele Report: Dmel\Ras85D[GD12553]

General Information

Symbol

Dmel\Ras85D^GD12553

Species

D. melanogaster

Name

FlyBase ID

FBal0208240

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{GD12553}

Also Known As

Ras^RNAi, UAS-Ras^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12553}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12553

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates colorectal cancer

modeled by Src42A^CA.UAS

(Cordero et al., 2014)

modeled by Src64B^UAS.cCa

(Cordero et al., 2014)

ameliorates neurofibromatosis

modeled by Nf1^JF01866

(Georganta et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Rewitz et al., 2009)

abnormal immune response | adult stage, with Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

decreased cell size | adult stage, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

decreased size | adult stage, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

increased mortality | adult stage | conditional, with Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Phenotype Manifest In

wing, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

wing cell, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

wing vein | absent, with Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

wing vein L2, with Scer\GAL4^salm-459.2

(Terriente-Félix and de Celis, 2009)

wing vein L3, with Scer\GAL4^salm-459.2

(Terriente-Félix and de Celis, 2009)

wing vein L4, with Scer\GAL4^salm-459.2

(Terriente-Félix and de Celis, 2009)

Detailed Description

Statement

Reference

Expression of Ras85D^GD12553 under the control of Scer\GAL4^Cg.PA leads to increased mortality upon infection with E. faecalis or S. saprophyticus when compared to controls.

(Iwashita et al., 2020)

Expression of Ras85D^GD12553 under the control of Scer\GAL4^salm.EPv leads to wings that fail to differentiate the veins and smaller wing cells.

(Vega-Cuesta et al., 2020)

Adults expressing Ras85D^GD12553 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Scer\GAL4^phm.PO-driven overexpression of Ras85D^GD12553 delays pupariation by 4.3 days.

(Rewitz et al., 2009)

Expression of Ras85D^GD12553 in the center of the wing using Scer\GAL4^salm-459.2 eliminates the veins L2, L3 and L4.

(Terriente-Félix and de Celis, 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12553

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ras85D^GD12553, Scer\GAL4^salm.EPv has decreased size | adult stage phenotype, enhanceable by Ras64B³/Ras64B³

(Vega-Cuesta et al., 2020)

Ras85D^GD12553, Scer\GAL4^salm.EPv has visible | adult stage phenotype, enhanceable by Ras64B³/Ras64B³

(Vega-Cuesta et al., 2020)

Ras85D^GD12553, Scer\GAL4^salm.EPv has decreased cell size | adult stage phenotype, enhanceable by Ras64B³/Ras64B³

(Vega-Cuesta et al., 2020)

Suppressed by

Statement

Reference

Ras85D^GD12553, Scer\GAL4^salm.EPv has visible | adult stage phenotype, suppressible by Ras64B^{V14.UAS.Tag:FLAG}, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Suppressor of

Statement

Reference

Ras85D^GD12553, Scer\GAL4^NP5130 is a suppressor of increased cell number | adult stage phenotype of Scer\GAL4^NP5130, aux^NIG.1107R

(Zhao et al., 2022)

Ras85D^GD12553, Scer\GAL4^Cg.PA is a suppressor of increased cell number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Ras85D^GD12553, Scer\GAL4^salm.EPv is a suppressor of increased size | adult stage phenotype of InR^A1325D.UAS, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Ras85D^GD12553, Scer\GAL4^salm.EPv is a suppressor of visible | adult stage phenotype of InR^A1325D.UAS, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Ras85D^GD12553, Scer\GAL4^NP5130 is a suppressor of hyperplasia phenotype of Scer\GAL4^NP5130, Src64B^UAS.cCa

(Cordero et al., 2014)

Ras85D^GD12553, Scer\GAL4^NP5130 is a suppressor of hyperplasia phenotype of Scer\GAL4^NP5130, Src42A^CA.UAS

(Cordero et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^GD12553, Scer\GAL4^salm.EPv has wing phenotype, enhanceable by Ras64B³/Ras64B³

(Vega-Cuesta et al., 2020)

Ras85D^GD12553, Scer\GAL4^salm.EPv has wing cell phenotype, enhanceable by Ras64B³/Ras64B³

(Vega-Cuesta et al., 2020)

NOT Enhanced by

Statement

Reference

Ras85D^GD12553, Scer\GAL4^salm-459.2 has wing vein L2 phenotype, non-enhanceable by osa^UAS.cCa

(Terriente-Félix and de Celis, 2009)

Ras85D^GD12553, Scer\GAL4^salm-459.2 has wing vein L3 phenotype, non-enhanceable by osa^UAS.cCa

(Terriente-Félix and de Celis, 2009)

Ras85D^GD12553, Scer\GAL4^salm-459.2 has wing vein L4 phenotype, non-enhanceable by osa^UAS.cCa

(Terriente-Félix and de Celis, 2009)

Suppressed by

Statement

Reference

Ras85D^GD12553, Scer\GAL4^salm.EPv has wing phenotype, suppressible by Ras64B^{V14.UAS.Tag:FLAG}, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Ras85D^GD12553, Scer\GAL4^salm.EPv has wing vein | absent phenotype, suppressible by Ras64B^{V14.UAS.Tag:FLAG}, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Suppressor of

Statement

Reference

Ras85D^GD12553, Scer\GAL4^NP5130 is a suppressor of adult posterior midgut epithelium phenotype of Scer\GAL4^NP5130, aux^NIG.1107R

(Zhao et al., 2022)

Ras85D^GD12553, Scer\GAL4^Cg.PA is a suppressor of embryonic/larval hemocyte | increased number | third instar larval stage phenotype of Gyc76C^UAS.Tag:MYC, Scer\GAL4^Cg.PA

(Iwashita et al., 2020)

Ras85D^GD12553, Scer\GAL4^salm.EPv is a suppressor of wing phenotype of InR^A1325D.UAS, Scer\GAL4^salm.EPv

(Vega-Cuesta et al., 2020)

Ras85D^GD12553, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Src64B^UAS.cCa

(Cordero et al., 2014)

Ras85D^GD12553, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4^NP5130, Src42A^CA.UAS

(Cordero et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of wing veins in animals expressing Ras85D^GD12553 under the control of Scer\GAL4^salm-459.2 is not altered by the expression of osa^Scer\UAS.cCa.

(Terriente-Félix and de Celis, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v28129

w¹¹¹⁸; P{GD12553}v28129

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12553

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ras85D^GD12553

Ras85D^RNAi

(Zhao et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

Open Close