FlyBase Allele Report: Dmel\Rho1[GD4726]

General Information

Symbol

Dmel\Rho1^GD4726

Species

D. melanogaster

Name

FlyBase ID

FBal0208281

Feature type

allele

Associated gene

Dmel\Rho1

Associated Insertion(s)

Carried in Construct

P{GD4726}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4726}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4726

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by RhoGEF2^RE.UAS, Raf^UAS.F179

(Khoo et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

abnormal cell shape | conditional | third instar larval stage, with Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

abnormal cell shape | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU

(Khoo et al., 2013)

lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

lethal - all die before end of P-stage, with Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

some die during pharate adult stage, with Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

visible | heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B

(Yan et al., 2009)

Phenotype Manifest In

actin filament & embryonic/larval tracheal system, with Scer\GAL4^btl.PS

(Massarwa et al., 2009)

adult abdomen | dorsal, with Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

adult cuticle | dorsal, with Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

cell projection, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

embryonic/larval hemocyte, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

eye-antennal disc | somatic clone | third instar larval stage, with Scer\GAL4^Tub.PU

(Khoo et al., 2013)

vacuole | increased number, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

wing | heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B

(Yan et al., 2009)

wing disc | conditional | third instar larval stage, with Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

wing hair | heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B

(Yan et al., 2009)

wing hair | increased number | heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B

(Yan et al., 2009)

Detailed Description

Statement

Reference

Hemocytes trans-migrate properly from the head to tail in embryos expressing Rho1^GD4726 under the control of Scer\GAL4^Pxn.PS. However, fewer of the tail hemocytes migrate anteriorly along the midline in the knock-down line than in wild-type.

Compared with wild-type, posterior hemocytes in Scer\GAL4^Pxn.PS>Rho1^GD4726 embryos exhibit severe reduction in cellular protrusion area. The anterior hemocytes of the same genotype also show a moderate reduction in cellular protrusion area. The number of vacuoles present in both the anterior and posterior hemocyte cell bodies is increased relative to wild-type.

(Verboon et al., 2015)

Eye-antennal disc clones expressing Rho1^GD4726 under the control of Scer\GAL4^tub.PU exhibit defects in differentiation and cell morphology, but clone size is not significantly affected as compared to control clones.

(Khoo et al., 2013)

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

(Schnorrer et al., 2010)

Expression of Rho1^GD4726 under the control of Scer\GAL4^ppl.PP results in animals with a dorsal cleft in the abdominal cuticle (most of these animals die in the puparium, with approximately 12% eclosing).

(Sekyrova et al., 2010)

Apical F-actin in the trachea disappears in embryos expressing Rho1^GD4726 under the control of Scer\GAL4^btl.PS.

(Massarwa et al., 2009)

Expression of Rho1^GD4726 in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4^ap-md544 by Scer\GAL80^ts.αTub84B) results in highly elongated apical-basal cell length in mutant cells compared with control cells.

(Widmann and Dahmann, 2009)

Cells in the wing expressing Rho1^GD4726 under the control of Scer\GAL4^ptc-559.1 (the flies also carry Scer\GAL80^ts.αTub84B but it has been inactivated by shifting to 29[o]C) often produce multiple hairs (cells without any hair are also seen).

Animals carrying Rho1^GD4726, Scer\GAL4^ptc-559.1 and Scer\GAL80^ts.αTub84B which have been shifted to 29[o]C for 26-36 hours to inactivate Scer\GAL80^ts.αTub84B and allow expression of Rho1^GD4726 under the control of Scer\GAL4^ptc-559.1 (in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) have pupal wings with cells that have a decreased height, increased cross sectional area and decreased F-actin. The dorsal and ventral layers of the wing separate basally leaving an internal hole.

(Yan et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4726

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rho1^GD4726, Scer\GAL4^ppl.PP has lethal | pharate adult stage phenotype, enhanceable by Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Rho1^GD4726, Scer\GAL4^ppl.PP has visible phenotype, enhanceable by Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Enhancer of

Statement

Reference

Rho1^GD4726, Scer\GAL4^ppl.PP is an enhancer of lethal | pharate adult stage phenotype of Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Rho1^GD4726, Scer\GAL4^ppl.PP is an enhancer of visible phenotype of Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Suppressor of

Statement

Reference

Rho1^GD4726, Scer\GAL4^Tub.PU is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Rho1^GD4726, Scer\GAL4^Tub.PU is a suppressor | somatic clone of abnormal developmental rate | somatic clone | larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rho1^GD4726, Scer\GAL4^ppl.PP has adult abdomen | dorsal phenotype, enhanceable by Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Enhancer of

Statement

Reference

Rho1^GD4726, Scer\GAL4^ppl.PP is an enhancer of adult abdomen | dorsal phenotype of Atf3^UAS.cSa, Scer\GAL4^ppl.PP

(Sekyrova et al., 2010)

Suppressor of

Statement

Reference

Rho1^GD4726, Scer\GAL4^Tub.PU is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Rho1^GD4726, Scer\GAL4^Tub.PU is a suppressor | somatic clone of eye photoreceptor cell | somatic clone | third instar larval stage phenotype of RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Rho1^GD4726, Scer\GAL4^Tub.PU is a suppressor | partially | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Rho1^GD4726, Scer\GAL4^Tub.PU is a suppressor | partially | somatic clone of eye photoreceptor cell | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, RhoGEF2^RE.UAS, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

NOT Suppressor of

Statement

Reference

Rho1^GD4726, Scer\GAL4^Tub.PU is a non-suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Rho1^GD4726, Scer\GAL4^Tub.PU is a non-suppressor | somatic clone of eye photoreceptor cell | somatic clone | third instar larval stage phenotype of Raf^UAS.F179, Scer\GAL4^Tub.PU

(Khoo et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Rho1^GD4726 suppresses the small clone size, differentiation and cell morphology defects seen in eye-antennal disc clones expressing RhoGEF2^RE.Scer\UAS under the control of Scer\GAL4^tub.PU.

Co-expression of Rho1^GD4726 suppresses the formation of undifferentiated clonal masses and partially suppresses the reduction of differentiation seen in eye-antennal disc clones expressing both RhoGEF2^RE.Scer\UAS and Raf^{Scer\UAS.F179} under the control of Scer\GAL4^tub.PU, and also suppresses the developmental delay shown by larvae containing these clones.

Co-expression of Rho1^GD4726 fails to suppress the ectopic differentiation of seen in eye-antennal disc clones expressing Raf^{Scer\UAS.F179} under the control of Scer\GAL4^tub.PU.

(Khoo et al., 2013)

Co-expression of Atf3^Scer\UAS.cSa and Rho1^GD4726 under the control of Scer\GAL4^ppl.PP results in a more severe phenotype than co-expression of either construct alone; no animals eclose and the defect in the dorsal abdomen becomes more severe.

(Sekyrova et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v12734

w¹¹¹⁸; P{GD4726}v12734/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4726

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rho1^GD4726

Rho1^RNAi

(Khoo et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (17)

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