Allele Dmel\Rho1^GD4726

General Information
Symbol	Dmel\Rho1^GD4726	Species	D. melanogaster
Name		FlyBase ID	FBal0208281
Feature type	allele	Associated gene	Dmel\Rho1

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..124 :125..205 :206..301
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD4726} (Yan et al., 2009, Dickson et al., 2007.7.18, Massarwa et al., 2009, Widmann and Dahmann, 2009, Sekyrova et al., 2010, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD4726
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective \| conditional \| third instar larval stage, with Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B (Widmann and Dahmann, 2009) lethal \| pharate adult stage, with Scer\GAL4^ppl.PP (Sekyrova et al., 2010) lethal \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^ppl.PP (Sekyrova et al., 2010) visible \| heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B (Yan et al., 2009)
Phenotype Manifest In
	actin filament & embryonic/larval tracheal system, with Scer\GAL4^btl.PS (Massarwa et al., 2009) adult abdomen \| dorsal, with Scer\GAL4^ppl.PP (Sekyrova et al., 2010) adult cuticle \| dorsal, with Scer\GAL4^ppl.PP (Sekyrova et al., 2010) wing \| heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B (Yan et al., 2009) wing disc \| conditional \| third instar larval stage, with Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B (Widmann and Dahmann, 2009) wing hair \| heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B (Yan et al., 2009) wing hair \| supernumerary \| heat sensitive, with Scer\GAL4^ptc-559.1, Scer\GAL80^ts.αTub84B (Yan et al., 2009)
Detailed Description
	Statement Reference Expression of Rho1[GD4726] under the control of Scer\GAL4[ppl.PP] results in animals with a dorsal cleft in the abdominal cuticle (most of these animals die in the puparium, with approximately 12% eclosing). (Sekyrova et al., 2010) Apical F-actin in the trachea disappears in embryos expressing Rho1[GD4726] under the control of Scer\GAL4[btl.PS]. (Massarwa et al., 2009) Expression of Rho1[GD4726] in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4[ap-md544] by Scer\GAL80[ts.αTub84B]) results in highly elongated apical-basal cell length in mutant cells compared with control cells. (Widmann and Dahmann, 2009) Cells in the wing expressing Rho1[GD4726] under the control of Scer\GAL4[ptc-559.1] (the flies also carry Scer\GAL80[ts.αTub84B] but it has been inactivated by shifting to 29[o]C) often produce multiple hairs (cells without any hair are also seen). Animals carrying Rho1[GD4726], Scer\GAL4[ptc-559.1] and Scer\GAL80[ts.αTub84B] which have been shifted to 29[o]C for 26-36 hours to inactivate Scer\GAL80[ts.αTub84B] and allow expression of Rho1[GD4726] under the control of Scer\GAL4[ptc-559.1] (in the presence of Dcr-2[Scer\UAS.cDa] to increase RNAi efficiency) have pupal wings with cells that have a decreased height, increased cross sectional area and decreased F-actin. The dorsal and ventral layers of the wing separate basally leaving an internal hole. (Yan et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 4726
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Rho1^GD4726, Scer\GAL4^ppl.PP has lethal \| pharate adult stage phenotype, enhanceable by Atf3^Scer\UAS.cSa, Scer\GAL4^ppl.PP (Sekyrova et al., 2010) Rho1^GD4726, Scer\GAL4^ppl.PP has visible phenotype, enhanceable by Atf3^Scer\UAS.cSa, Scer\GAL4^ppl.PP (Sekyrova et al., 2010)
Enhancer of
Statement Reference Rho1^GD4726, Scer\GAL4^ppl.PP is an enhancer of lethal \| pharate adult stage phenotype of Atf3^Scer\UAS.cSa, Scer\GAL4^ppl.PP (Sekyrova et al., 2010) Rho1^GD4726, Scer\GAL4^ppl.PP is an enhancer of visible phenotype of Atf3^Scer\UAS.cSa, Scer\GAL4^ppl.PP (Sekyrova et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Rho1^GD4726, Scer\GAL4^ppl.PP has adult abdomen \| dorsal phenotype, enhanceable by Atf3^Scer\UAS.cSa, Scer\GAL4^ppl.PP (Sekyrova et al., 2010)
Enhancer of
Statement Reference Rho1^GD4726, Scer\GAL4^ppl.PP is an enhancer of adult abdomen \| dorsal phenotype of Atf3^Scer\UAS.cSa, Scer\GAL4^ppl.PP (Sekyrova et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Atf3[Scer\UAS.cSa] and Rho1[GD4726] under the control of Scer\GAL4[ppl.PP] results in a more severe phenotype than co-expression of either construct alone; no animals eclose and the defect in the dorsal abdomen becomes more severe. (Sekyrova et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
VDRC	v12734 w¹¹¹⁸; P{GD4726}v12734/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 4726
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Rho1^GD4726
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Sekyrova et al., 2010, Development 137(1): 141--150 Interaction between Drosophila bZIP proteins Atf3 and Jun prevents replacement of epithelial cells during metamorphosis. [FBrf0209550] Massarwa et al., 2009, Dev. Cell 16(6): 877--888 Apical secretion in epithelial tubes of the Drosophila embryo is directed by the Formin-family protein Diaphanous. [FBrf0208270] Widmann and Dahmann, 2009, J. Cell Sci. 122(9): 1362--1373 Dpp signaling promotes the cuboidal-to-columnar shape transition of Drosophila wing disc epithelia by regulating Rho1. [FBrf0207672] Yan et al., 2009, Dev. Biol. 333(1): 186--199 Rho1 has multiple functions in Drosophila wing planar polarity. [FBrf0208582]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\Rho1GD4726

Allele Dmel\Rho1^GD4726