Expression of RxGD4076 under the control of Scer\GAL4insc-Mz1407 results in a significant decrease in the number of mushroom body neuroblasts and Kenyon cells in the mushroom body at 72 h after puparium formation, a decrease in the number of mushroom body GMCs at the third instar stage, a reduction in proliferation of mushroom body neuroblasts and GMCs in late third instar brains, and a decrease in third instar mushroom body neuroblast and GMC size, as compared to controls.
Expression under the control of Scer\GAL41407 results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.
Adults expressing RxGD4076 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or late pupal lethality.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 30-40% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 30-40% or 90-100% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.