Expression of Sema1a^GD2504 under the control of Scer\GAL4^VGlut-OK371 results in defective walking pattern (uncoordinated walk with leg dragging) and impaired innervation of the leg muscles in proximal femur (failed defasciculation or insufficient elongation of axon branches) as well as distal tibia (increase in the number of branches that exit the main nerve). Similarly, expression under the Scer\GAL4^{VGlut.VGN6341} driver results in axon mis-projection phenotypes in the leg muscles - lack of innervation of the muscles of the femur and excessive innervation of the muscles of the tibia, expression under Scer\GAL4^GMR60B11 also leads to lack of innervation in the femur.

Adults expressing Sema-1a^GD14307 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.