FlyBase Allele Report: Dmel\Src64B[GD12263]

General Information

Symbol

Dmel\Src64B^GD12263

Species

D. melanogaster

Name

FlyBase ID

FBal0208624

Feature type

allele

Associated gene

Dmel\Src64B

Associated Insertion(s)

Carried in Construct

P{GD12263}

Also Known As

UAS-src64B-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12263}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12263

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS

(Ma et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

decreased occurrence of cell division | adult stage | conditional, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Phenotype Manifest In

adult midgut, with Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

eye, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Detailed Description

Statement

Reference

Expression of Src64B^GD12263 under the control of Scer\GAL4^GMR.PF at 29[o]C results in male eyes that are 42% smaller than controls.

(Pechkovsky et al., 2013)

Adults expressing Src64B^GD12263 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12263

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.PU/Src64B^GD12263, Scer\GAL80^ts.αTub84B is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^FRT.Act5C/Src64B^GD12263 is a suppressor of lethal - all die during larval stage phenotype of Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2013)

Src64B^GD12263, Scer\GAL4^FRT.Act5C is a suppressor of lethal - all die during larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C

(Ma et al., 2013)

Src64B^GD12263, Scer\GAL4^FRT.Act5C is a suppressor of increased cell growth rate phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2013)

Src64B^GD12263, Scer\GAL4^FRT.Act5C is a suppressor of abnormal neuroanatomy phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2013)

Src64B^GD12263, Scer\GAL4^FRT.Act5C is a suppressor of neoplasia phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2013)

Src64B^GD12263, Scer\GAL4^GMR.PF is a suppressor | partially of visible | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Src64B^GD12263, Scer\GAL4^GMR.PF is a suppressor | partially of abnormal size | adult stage phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Src64B^GD12263, Scer\GAL80^ts.αTub84B is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Src64B^GD12263, Scer\GAL4^NP7397 is a non-suppressor of increased occurrence of cell division | adult stage phenotype of Ret^{UAS.Tag:FLAG,Tag:polyHis}, Scer\GAL4^NP7397

(Perea et al., 2017)

Other

Statement

Reference

Scer\GAL4^NP7397, Src42A^GD10610, Src64B^GD12263 has abnormal immune response | adult stage phenotype

(Kohlmaier et al., 2015)

Scer\GAL4^NP7397, Src42A^GD10610, Src64B^GD12263 has decreased cell number | adult stage phenotype

(Kohlmaier et al., 2015)

Scer\GAL4^NP7397, Src42A^GD10610, Src64B^GD12263 has decreased occurrence of cell division | adult stage | conditional phenotype

(Kohlmaier et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Src64B^GD12263, Scer\GAL4^FRT.Act5C is a suppressor of larval ventral nerve cord phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, l(2)gl⁴

(Ma et al., 2013)

Src64B^GD12263, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

NOT Suppressor of

Statement

Reference

Src64B^GD12263, Scer\GAL4^NP7397 is a non-suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Ret^{UAS.Tag:FLAG,Tag:polyHis}, Scer\GAL4^NP7397

(Perea et al., 2017)

Other

Statement

Reference

Scer\GAL4^NP7397, Src42A^GD10610, Src64B^GD12263 has adult midgut phenotype

(Kohlmaier et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing Ret^{Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6} driven by Scer\GAL4^esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is not suppressed by co-expression of Src64B^GD12263.

(Perea et al., 2017)

Loss of Src64B, through expression of Src64B^GD12263, dramatically suppresses tumour cell invasion into the ventral nerve cord in Ras85D^V12.Scer\UAS/l(2)gl⁴ mutants and enables the animals to survive to the pupal stage, whereas the tumour size remains largely unaffected.

(Ma et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of Src64B^GD12263 partially suppresses the eye phenotypes seen in males when Zzzz\E4orf4^Scer\UAS.cPa is expressed under the control of Scer\GAL4^GMR.PF at 29[o]C. Eye size is restored to 63% of controls.

(Pechkovsky et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v35252

w¹¹¹⁸ P{GD12263}v35252

Notes on Origin

Discoverer