Allele Dmel\Stim^GD16187

General Information
Symbol	Dmel\Stim^GD16187	Species	D. melanogaster
Name		FlyBase ID	FBal0208646
Feature type	allele	Associated gene	Dmel\Stim

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms lethal
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..347
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD16187} (Venkiteswaran and Hasan, 2009, Dickson et al., 2007.7.18, Agrawal et al., 2010, Mummery-Widmer et al., 2009, Sadaf et al., 2012, Tsubouchi et al., 2012)
Associated Sequence Features	dsRNA-GD16187
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Dcr-2^Scer\UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW (Tsubouchi et al., 2012) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	trichogen cell \| supernumerary, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Flies expressing Stim[GD16187] under the control of Scer\GAL4[Trh.PB] show normal flight ability in a cylinder drop test. However, the flies show increased spontaneous firing from the dorsal longitudinal indirect flight muscles compared to wild type. (Sadaf et al., 2012) Expression under the control of Scer\GAL4[pnr-MD237] results in loss of bristles on the notum in 50-60% of the Scer\GAL4[pnr-MD237] expression domain. Expression under the control of Scer\GAL4[pnr-MD237] results in duplication of bristle hairs on the notum in 0-10% or 10-20% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. (Mummery-Widmer et al., 2009) No obvious changes are observed in Stim[GD16187] larvae. They are viable and pupate normally. Adult flies with pan-neural expression of Stim[GD16187] (under the control of Scer\GAL4[elav-C155]) exhibit defective wing posture with significant loss of flight, as seen in the 'cylinder drop' test assay. Stim[GD16187] knockdown (under the control of Scer\GAL4[elav-C155]) results in a complete loss of flight. Nonfliers with pan-neural expression of Stim[GD16187] selected from the cylinder drop test are either unable to initiate rhythmic action potentials in response to an air-puff stimulus or exhibit unsustained (<5s) and arrhythmic flight patters. Knockdown of Stim[GD16187] in glutamatergic neurons leads to a milder change in flight patterns compared with pan-neural knockdown. Recordings from resting DLMs of these flies reveals a high arrhythmic spontaneous firing rate of action potentials, the frequency of which is significantly higher than wild-type. (Venkiteswaran and Hasan, 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 16187
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v47074 w¹¹¹⁸ P{GD16187}v47074 v47073 w¹¹¹⁸; P{GD16187}v47073
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 16187
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Stim^GD16187
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Sadaf et al., 2012, PLoS ONE 7(9): e46405 Synaptic Activity in Serotonergic Neurons Is Required for Air-Puff Stimulated Flight in Drosophila melanogaster. [FBrf0219558] Agrawal et al., 2010, J. Neurosci. 30(4): 1301--1313 Inositol 1,4,5-trisphosphate receptor and dSTIM function in Drosophila insulin-producing neurons regulates systemic intracellular calcium homeostasis and flight. [FBrf0209847] Venkiteswaran and Hasan, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(25): 10326--10331 Intracellular Ca2+ signaling and store-operated Ca2+ entry are required in Drosophila neurons for flight. [FBrf0208231]
Supplementary material	Tsubouchi et al., 2012, Curr. Biol. 22(22): Tables S1 and S2. RNAi Lines Tested for Screening and Candidate Channel Genes, Related to Figure 5. [FBrf0220657] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\StimGD16187

Allele Dmel\Stim^GD16187