Expressing Tango1GD956 under the control of Scer\GAL4SPARC-MI00329-GAL4 leads to impaired protein secretion in third instar larval adipocytes, as evidenced by the retention of the secretion marker secr.GFP.
The expression of Tango1GD956 under the control of Scer\GAL4SPARC-MI00329-GAL4 (and Dicer-2, for a more efficient RNAi) in the larval fat body leads to defects in general secretion, as shown by the aberrant intracellular accumulation of Vkg.GFP (collagen IV), Trol.GFP, secr.GFP and Vkg.GFP.
The secretory cells (PR cells) of the proventriculus of larvae expressing Tango1GD956 show disrupted secretion, are enlarged, and display mislocalization of apical secretory components and loss of secretory vesicles, compared to wild type.
Knockdown of Tango1 in the fat body, through expression of Tango1GD956 under the control of Scer\GAL4Cg.PA causes retention of Avic\GFPvkg-G454 in fat body cells. Avic\GFPvkg-G454 accumulates in growing intracellular aggregates. These eventually coalesce and occupy most of the cytoplasm between the lipid droplets, affecting cell viability. These larvae lack Avic\GFPvkg-G454 in the basement membranes of other organs, such as the wing disc.
Adults expressing Tango1GD956 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.
Adults expressing Tango1GD956 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.