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General Information
Symbol
Dmel\Tango1GD956
Species
D. melanogaster
Name
FlyBase ID
FBal0208723
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Tango1.RNAiVDRC.v21594
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expressing Tango1GD956 under the control of Scer\GAL4SPARC-MI00329-GAL4 leads to impaired protein secretion in third instar larval adipocytes, as evidenced by the retention of the secretion marker secr.GFP.

The expression of Tango1GD956 under the control of Scer\GAL4SPARC-MI00329-GAL4 (and Dicer-2, for a more efficient RNAi) in the larval fat body leads to defects in general secretion, as shown by the aberrant intracellular accumulation of Vkg.GFP (collagen IV), Trol.GFP, secr.GFP and Vkg.GFP.

The secretory cells (PR cells) of the proventriculus of larvae expressing Tango1GD956 show disrupted secretion, are enlarged, and display mislocalization of apical secretory components and loss of secretory vesicles, compared to wild type.

Knockdown of Tango1 in the fat body, through expression of Tango1GD956 under the control of Scer\GAL4Cg.PA causes retention of Avic\GFPvkg-G454 in fat body cells. Avic\GFPvkg-G454 accumulates in growing intracellular aggregates. These eventually coalesce and occupy most of the cytoplasm between the lipid droplets, affecting cell viability. These larvae lack Avic\GFPvkg-G454 in the basement membranes of other organs, such as the wing disc.

Adults expressing Tango1GD956 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

Adults expressing Tango1GD956 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Tango1GD956
Name Synonyms
Secondary FlyBase IDs
    References (11)