FlyBase Allele Report: Dmel\mrn[GD4089]

General Information

Symbol

Dmel\mrn^GD4089

Species

D. melanogaster

Name

FlyBase ID

FBal0208772

Feature type

allele

Associated gene

Dmel\mrn

Associated Insertion(s)

Carried in Construct

P{GD4089}

Key Links

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD4089}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mrn

Associated Sequence Features

dsRNA-GD4089

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

decreased cell number, with Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

decreased cell size, with Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

increased cell death, with Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

visible, with Scer\GAL4^en.PU

(Villicaña et al., 2013)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

posterior crossvein, with Scer\GAL4^en.PU

(Villicaña et al., 2013)

wing, with Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

wing, with Scer\GAL4^en.PU

(Villicaña et al., 2013)

wing disc, with Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

wing vein | ectopic, with Scer\GAL4^en.PU

(Villicaña et al., 2013)

Detailed Description

Statement

Reference

Expression of mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096 results in wings which are smaller than normal, have a "bent-out" shape and which contain extra wing veins. Wing cell size is smaller than normal, and the total number of cells in the wing is reduced compared to wild type. BrdU incorporation is normal in wing discs expressing mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096, but the number of apoptotic cells is increased compared to controls.

Expression of mrn^GD4089 under the control of Scer\GAL4^en.PU results in wings which are smaller than normal, have a highly reduced posterior crossvein, and contain extra wing veins (which can be formed outside the domain of Scer\GAL4^en.PU expression).

(Villicaña et al., 2013)

Adults expressing mrn^GD4089 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4089

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, mrn^GD4089 has visible phenotype, enhanceable by p53^Ct.GUS/Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Scer\GAL4^Bx-MS1096, mrn^GD4089 has increased cell death phenotype, enhanceable by p53^Ct.GUS/Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, mrn^GD4089, p53^Ct.GUS has increased cell death phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Scer\GAL4^Bx-MS1096, mrn^GD4089, p53^Ct.GUS has increased cell death phenotype, suppressible by bsk^GD10555/Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Scer\GAL4^Bx-MS1096, mrn^GD4089 has increased cell death phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Other

Statement

Reference

BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096, mrn^GD4089 has visible phenotype

(Villicaña et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, mrn^GD4089 has wing disc phenotype, enhanceable by p53^Ct.GUS/Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Scer\GAL4^Bx-MS1096, mrn^GD4089 has wing phenotype, enhanceable by p53^Ct.GUS/Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Suppressed by

Statement

Reference

mrn^GD4089 has wing disc phenotype, suppressible by BacA\p35^UAS.cUa/Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Scer\GAL4^Bx-MS1096, mrn^GD4089, p53^Ct.GUS has wing disc phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of p53^Ct.GUS enhances the wing defects seen in flies expressing mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096.

Co-expression of p53^Ct.GUS enhances the increased apoptosis seen in the wing discs of animals expressing mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096.

The massive apoptosis seen in wing discs co-expressing p53^Ct.GUS and mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096 is practically abolished by co-expression of bsk^GD10555.

(Villicaña et al., 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of mrn^GD4089 and BacA\p35^Scer\UAS.cUa under the control of Scer\GAL4^Bx-MS1096 results in wings containing a large blister.

Co-expression of BacA\p35^Scer\UAS.cUa suppresses the increased number of apoptotic cells seen in the wing discs of animals expressing mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096.

The massive apoptosis seen in wing discs co-expressing p53^Ct.GUS and mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096 is suppressed by co-expression of BacA\p35^Scer\UAS.cUa.

(Villicaña et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v12596

w¹¹¹⁸; P{GD4089}v12596

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Crossreferences

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4089

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tfb2^GD4089

mrn^GD4089

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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