FlyBase Allele Report: Dmel\Top2[GD4570]

General Information

Symbol

Dmel\Top2^GD4570

Species

D. melanogaster

Name

FlyBase ID

FBal0208817

Feature type

allele

Associated gene

Dmel\Top2

Associated Insertion(s)

Carried in Construct

P{GD4570}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4570}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4570

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^Act.PU

(Mengoli et al., 2014)

decreased size | pharate adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

lethal - all die before end of first instar larval stage, with Scer\GAL4^Act5C.PI

(Ramos et al., 2011)

lethal - all die before end of P-stage, with Scer\GAL4^en.PU

(Yang et al., 2021)

lethal - all die during larval stage, with Scer\GAL4^Act.PU

(Mengoli et al., 2014)

lethal - all die during P-stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chromosome | third instar larval stage, with Scer\GAL4^Act.PU

(Mengoli et al., 2014)

embryonic/larval salivary gland | larval stage | male, with Scer\GAL4^Act.PU

(Mengoli et al., 2014)

eye | absent | pharate adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

head | pharate adult stage, with Scer\GAL4^ey.PU

(Yang et al., 2021)

larval brain | third instar larval stage, with Scer\GAL4^Act.PU

(Mengoli et al., 2014)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

polytene chromosome | larval stage | male, with Scer\GAL4^Act.PU

(Mengoli et al., 2014)

Detailed Description

Statement

Reference

Dead adult pharates expressing Top2^GD4570 under the control of Scer\GAL4^ey.PU show loss of eyes and severe disruption of head structures.

(Yang et al., 2021)

The brains of third instar larvae expressing Top2^GD4570 under the control of Scer\GAL4^Act.PU display significantly decreased mitotic index compared to controls, the chromosomes are often undercondensed, elongated or fragmented and most of the metaphasic cells are poly- or hyperploid.

Male larvae expressing Top2^GD4570 under the control of Scer\GAL4^Act.PU display defects in the morphology of the polytene chromosome X in larval salivary glands. The X chromosome appears bloated and shows partial or complete loss of its typical banding pattern.

(Mengoli et al., 2014)

Expression of Top2^GD4570 under the control of Scer\GAL4^arm.PS results in a decrease in gypsy insulator function, manifested by a change in body colour from yellow to black in y² mutants.

Expression of Top2^GD4570 in the prospective wing margin region of the developing wing under the control of Scer\GAL4^bbg-C96 results in a decrease in gypsy insulator function and suppresses the ct⁶ phenotype back to wild-type.

(Ramos et al., 2011)

Adults expressing Top2^GD4570 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4570

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Top2^GD4570/Scer\GAL4^arm.PS is a suppressor of abnormal body color phenotype of y²

(Ramos et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^bbg-C96/Top2^GD4570 is a suppressor of wing phenotype of ct⁶

(Ramos et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v30625

w¹¹¹⁸; P{GD4570}v30625

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4570

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Top2^GD4570

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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