Expression of Treh^GD5118 driven in adult neurons using Scer\GAL4^nSyb.PS in combination with GAL80ts does not significantly affect lifespan; knockdown in adult glia (with Scer\GAL4^repo.PU and GAL80ts) significantly shortens lifespan compared to controls and leads to neuronal cell death.

The brains of late third instar larvae expressing Treh^GD5118 under the control of Scer\GAL4^c768 do not have a lamina. Defects in the outer proliferation center (OPC) neuroepithelium are first seen around the mid third larval instar stage, with the neuroepithelium beginning to disintegrate and cells having an abnormal morphology. Very few neuroepithelial cells remain in the optic lobe by the late third instar stage, while some large, rounded cells expressing neuroblast markers are seen in the medulla cortex.

The brains of late third instar larvae expressing Treh^GD5118 under the control of Scer\GAL4^C855a do not have a lamina and have an underdeveloped medulla. The OPC neuroepithelium begins to disintegrate around the mid third larval instar stage.

The brains of late third instar larvae expressing Treh^GD5118 under the control of Scer\GAL4^NP3605 do not have a lamina and have an underdeveloped medulla.

Clones expressing Treh^GD5118 under the control of Scer\GAL4^Act.PU which remain in the OPC neuroepithelium do not change epithelial cell identity. Clones are more frequently found in the medulla cortex, suggesting that mutant cells originating in the OPC neuroepithelium are extruded basally into the medulla. Cells within clones in the medulla are rounded and appear (by analysis of marker expression and cell proliferation) to correspond to neuroblasts with a reversal of apical-basal polarity compared to normal medulla neuroblasts. The mutant neuroblasts are rarely seen in anaphase or telophase and the clones generate only a few neurons.

Adults expressing Treh^GD5118 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in defects in locomotion.

Expression under the control of Scer\GAL4^pnr-MD237 may result in lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 40-50% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.