FlyBase Allele Report: Dmel\Tsc1[GD11836]

General Information

Symbol

Dmel\Tsc1^GD11836

Species

D. melanogaster

Name

FlyBase ID

FBal0208859

Feature type

allele

Associated gene

Dmel\Tsc1

Associated Insertion(s)

Carried in Construct

P{GD11836}

Also Known As

UAS-TSC1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11836}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11836

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased size | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2023)

increased size | spermatogenesis, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^GMR.PU

(Li et al., 2023)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

eye, with Scer\GAL4^GMR.PU

(Li et al., 2023)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

spermatocyte, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

Detailed Description

Statement

Reference

Adults expressing Tsc1^GD11836 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in mild thorax closure defects.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11836

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Tsc1^GD11836 has visible | adult stage phenotype, enhanceable by Ufl1^HMJ30245/Uba5^HMS01352/Scer\GAL4^GMR.PU

(Li et al., 2023)

Scer\GAL4^GMR.PU, Tsc1^GD11836 has increased size | adult stage phenotype, enhanceable by Ufl1^HMJ30245/Uba5^HMS01352/Scer\GAL4^GMR.PU

(Li et al., 2023)

Enhancer of

Statement

Reference

Tsc1^GD11836, Scer\GAL4^repo.PU is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Tsc1^GD11836, Scer\GAL4^repo.PU is an enhancer of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

NOT Suppressor of

Statement

Reference

Tsc1^GD11836, Scer\GAL4^NP0001 is a non-suppressor of increased cell size phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, Tsc1^GD11836 has eye phenotype, enhanceable by Ufl1^HMJ30245/Uba5^HMS01352/Scer\GAL4^GMR.PU

(Li et al., 2023)

Enhancer of

Statement

Reference

Tsc1^GD11836, Scer\GAL4^repo.PU is an enhancer of glial cell | third instar larval stage | increased number phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Tsc1^GD11836, Scer\GAL4^repo.PU is an enhancer of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

NOT Suppressor of

Statement

Reference

Tsc1^GD11836, Scer\GAL4^NP0001 is a non-suppressor of adult midgut epithelium phenotype of Atg9^HMS01246, Scer\GAL4^NP0001

(Wen et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Tsc1^GD11836 does not suppress the increased midgut cell size resulting from the expression of Atg9^HMS01246 under the control of Scer\GAL4^{Myo31DF-NP0001}.

(Wen et al., 2017)

Expression of Tsc1^GD11836 enhances the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU.

(Read et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22252

w¹¹¹⁸; P{GD11836}v22252/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11836

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Tsc1^GD11836

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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