FlyBase Allele Report: Dmel\Usp47[GD10642]

General Information

Symbol

Dmel\Usp47^GD10642

Species

D. melanogaster

Name

FlyBase ID

FBal0208913

Feature type

allele

Associated gene

Dmel\Usp47

Associated Insertion(s)

Carried in Construct

P{GD10642}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10642}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10642

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^Bx-MS1096

(Shi et al., 2015)

Phenotype Manifest In

wing blade, with Scer\GAL4^Bx-MS1096

(Shi et al., 2015)

wing margin bristle, with Scer\GAL4^Bx-MS1096

(Shi et al., 2015)

Detailed Description

Statement

Reference

Expression of Usp47^GD10642 RNAi under the control of Scer\GAL4^Bx-MS1096 results in wing margin bristle defects. Co-expression with UAS-Dicer2 (to enhance RNAi efficiency) leads to a severe wing malformation that includes wing margin bristle defects.

(Shi et al., 2015)

Expression of Ubp64E^GD10642 under the control of Scer\GAL4^bbg-C96 does not result in a wing phenotype.

Expression of Ubp64E^GD10642 under the control of Scer\GAL4^dpp.PU does not result in a bristle phenotype.

(Zhang et al., 2012)

Adults expressing Usp47^GD10642 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10642

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^GD8339

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^KK101471

(Ashton-Beaucage et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^salm.EPv/Usp47^GD10642 is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^salm.EPv, rl^Sem.S.UAS

(Ashton-Beaucage et al., 2016)

Other

Statement

Reference

Scer\GAL4^en-e16E, Ubc6^GD13294, Usp47^GD10642 has some die during larval stage phenotype

(Ashton-Beaucage et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing blade phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^GD8339

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing vein phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^GD8339

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing blade phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^KK101471

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv, Usp47^GD10642, rl^Sem.S.UAS has wing vein phenotype, enhanceable by Scer\GAL4^salm.EPv/poe^KK101471

(Ashton-Beaucage et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^salm.EPv/Usp47^GD10642 is a suppressor | partially of wing blade phenotype of Scer\GAL4^salm.EPv, rl^Sem.S.UAS

(Ashton-Beaucage et al., 2016)

Scer\GAL4^salm.EPv/Usp47^GD10642 is a suppressor | partially of wing vein | ectopic phenotype of Scer\GAL4^salm.EPv, rl^Sem.S.UAS

(Ashton-Beaucage et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Usp47^GD10642 and Ubc6^GD13294 under the control of Scer\GAL4^en-e16E leads to extensive larval lethality and the wing discs from larvae that can be recovered have a reduced size compared to controls.

The formation of ectopic wein material in the adult wing characteristic for flies expressing rl^{Sem.S.Scer\UAS} under the control of Scer\GAL4^salm.EPv is partially suppressed by co-expression of either Usp47^GD10642 or Usp47^NIG.5486R. The ameliorated phenotype of Scer\GAL4^salm.EPv,rl^{Sem.S.Scer\UAS};Usp47^GD10642 flies can be worsened again by co-expression of either poe^GD8339 or poe^KK101471 : the severity of their ectopic venation phenotype is comparable to that of flies expressing rl^{Sem.S.Scer\UAS} only.

(Ashton-Beaucage et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v26027

w¹¹¹⁸; P{GD10642}v26027

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10642

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ubp64E^GD10642

Usp47^GD10642

Usp47ⁱ²

(Kassel et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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