FlyBase Allele Report: Dmel\Uch[GD11265]

General Information

Symbol

Dmel\Uch^GD11265

Species

D. melanogaster

Name

FlyBase ID

FBal0208915

Feature type

allele

Associated gene

Dmel\Uch

Associated Insertion(s)

Carried in Construct

P{GD11265}

Also Known As

UAS-dUCH-IR, UAS-dUCH.IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11265}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11265

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Tran et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates metal metabolism disorder

modeled by ATP7^UAS.Tag:FLAG

(Zlatic et al., 2018)

ameliorates Parkinson's disease 6

modeled by Pink1^B9

(Ham et al., 2021)

ameliorates Parkinson's disease 2

modeled by park¹

(Ham et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^Ddc.PL

(Tran et al., 2018)

abnormal locomotor behavior | adult stage, with Scer\GAL4^ple.PF

(Tran et al., 2018)

abnormal locomotor behavior | larval stage, with Scer\GAL4^ple.PF

(Tran et al., 2018)

abnormal size | larval stage, with Scer\GAL4^Toll-6-D42

(Tran et al., 2018)

decreased cell number | adult stage | progressive, with Scer\GAL4^ple.PF

(Tran et al., 2018)

decreased cell number | larval stage, with Scer\GAL4^ple.PF

(Tran et al., 2018)

lethal - all die before end of pupal stage, with Scer\GAL4^Act5C.PI

(Tran et al., 2018)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

chaeta, with Scer\GAL4^pnr-MD237

(Tran et al., 2018)

dopaminergic PPL2ab neuron | progressive, with Scer\GAL4^ple.PF

(Tran et al., 2018)

dopaminergic PPM2 neuron | progressive, with Scer\GAL4^ple.PF

(Tran et al., 2018)

dopaminergic PPM3 neuron | progressive, with Scer\GAL4^ple.PF

(Tran et al., 2018)

eye, with Scer\GAL4^GMR.PS

(Tran et al., 2018)

larval dopaminergic DL1 neuron, with Scer\GAL4^ple.PF

(Tran et al., 2018)

larval VUM neuron | progressive, with Scer\GAL4^ple.PF

(Tran et al., 2018)

neuromuscular junction | larval stage, with Scer\GAL4^Toll-6-D42

(Tran et al., 2018)

synapse | larval stage, with Scer\GAL4^Toll-6-D42

(Tran et al., 2018)

type I bouton | larval stage, with Scer\GAL4^Toll-6-D42

(Tran et al., 2018)

wing vein, with Scer\GAL4^Bx-MS1096

(Tran et al., 2018)

Detailed Description

Statement

Reference

The expression of Uch^GD11265 under the control of Scer\GAL4^ple.PF results in locomotor dysfunction and deficit in both larvae and adults, loss of larval DL1 cluster DA neurons, but not DM1a, DM1b, DM2, DL2a or DL2b cluster DA neurons, and loss of adult PPM2, PPM3 (only from the PPM3b sub-cluster), PPL2ab and VUM cluster DA neurons, as compared to controls. The expression of Uch^GD11265 also results in pupal lethality (driven by Scer\GAL4^Act5C.PI), in a rough eye phenotype (driven by Scer\GAL4^GMR.PS), in an abnormal vein pattern (driven by Scer\GAL4^Bx-MS1096), in abnormal bristle and hair patterns (driven by Scer\GAL4^pnr-MD237), in a progressive locomotor deficit (driven by Scer\GAL4^Ddc.PL), and in an abnormal morphology larval neuromuscular junction morphology, namely longer branches and fewer and bigger type Ib boutons (driven by Scer\GAL4^Toll-6-D42), as compared to controls.

(Tran et al., 2018)

No phenotype is observed when Uch^GD11265 is expressed using Scer\GAL4^sqh.PW or Scer\GAL4^elav.PU.

(Tsou et al., 2012)

Adults expressing Uch^GD11265 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development