Allele Dmel\Unc-89^GD14839

General Information
Symbol	Dmel\Unc-89^GD14839	Species	D. melanogaster
Name		FlyBase ID	FBal0208946
Feature type	allele	Associated gene	Dmel\Unc-89

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..328
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD14839} (Dickson et al., 2007.7.18, Schnorrer et al., 2010, Mummery-Widmer et al., 2009, Katzemich et al., 2012)
Associated Sequence Features	dsRNA-GD14839
Cytology
Phenotypic Data
Phenotypic Class
	flightless, with Scer\GAL4^Mef2.PR (Katzemich et al., 2012) flightless, with Scer\GAL4^UH3 (Katzemich et al., 2012) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	H zone \| adult stage, with Scer\GAL4^Mef2.PR (Katzemich et al., 2012) H zone \| adult stage, with Scer\GAL4^UH3 (Katzemich et al., 2012) indirect flight muscle, with Scer\GAL4^Mef2.PR (Schnorrer et al., 2010) indirect flight muscle \| adult stage, with Scer\GAL4^Mef2.PR (Katzemich et al., 2012) indirect flight muscle \| adult stage, with Scer\GAL4^UH3 (Katzemich et al., 2012) M band, with Scer\GAL4^Mef2.PR (Schnorrer et al., 2010) myofibril \| adult stage, with Scer\GAL4^Mef2.PR (Katzemich et al., 2012) myofibril \| adult stage, with Scer\GAL4^UH3 (Katzemich et al., 2012) sarcomere \| pharate adult stage, with Scer\GAL4^Mef2.PR (Katzemich et al., 2012) striated muscle thin filament \| adult stage, with Scer\GAL4^Mef2.PR (Katzemich et al., 2012)
Detailed Description
	Statement Reference Animals expressing Unc-89[GD14839] under the control of Scer\GAL4[Mef2.PR] have no regular sarcomeres in the indirect flight muscles at 72hours after pupariation formation (APF), in contrast to what is seen in wild type. Adults expressing Unc-89[GD14839] under the control of either Scer\GAL4[Mef2.PR] or Scer\GAL4[UH3] are flightless, although they show a normal jump response. Myofibrils in the indirect flight muscle are narrower than normal and ultrastructurally, the H-zone is shifted away from the middle of the sarcomere. The thick filaments are asymmetrical in indirect flight muscles expressing Unc-89[GD14839] under the control of Scer\GAL4[Mef2.PR], with some bare zones far from the middle of the sarcomere. The polarity of the thin filaments follows that of the thick filaments, resulting in abnormally long or short thin filaments. Thin filaments sometimes extend into the H-zone. Larvae expressing Unc-89[GD14839] under the control of Scer\GAL4[Mef2.PR] show slightly reduced crawling ability compared to wild type, whereas expression under the control of Scer\GAL4[UH3] is normal. (Katzemich et al., 2012) Adults expressing Unc-89[GD14839] under the control of Scer\GAL4[Mef2.PR] have indirect flight muscles with no visible M-line. (Schnorrer et al., 2010)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 14839
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v29412 w¹¹¹⁸; P{GD14839}v29412 v29413 w¹¹¹⁸; P{GD14839}v29413/TM3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 14839
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Unc-89^GD14839
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Katzemich et al., 2012, J. Cell Sci. 125(14): 3367--3379 The function of the M-line protein obscurin in controlling the symmetry of the sarcomere in the flight muscle of Drosophila. [FBrf0219368] Schnorrer et al., 2010, Nature 464(7286): 287--291 Systematic genetic analysis of muscle morphogenesis and function in Drosophila. [FBrf0210226]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\Unc-89GD14839

Allele Dmel\Unc-89^GD14839