FlyBase Allele Report: Dmel\Vap33[GD3990]

General Information

Symbol

Dmel\Vap33^GD3990

Species

D. melanogaster

Name

FlyBase ID

FBal0208959

Feature type

allele

Associated gene

Dmel\Vap33

Associated Insertion(s)

Carried in Construct

P{GD3990}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3990}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3990

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

abnormal neuroanatomy | larval stage, with Scer\GAL4^C57

(Forrest et al., 2013)

abnormal neurophysiology | adult stage, with Scer\GAL4^GMR.PF

(Yadav et al., 2018)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

NMJ bouton, with Scer\GAL4^elav.PU

(Forrest et al., 2013)

NMJ bouton | larval stage, with Scer\GAL4^C57

(Forrest et al., 2013)

retina | adult stage, with Scer\GAL4^GMR.PF

(Yadav et al., 2018)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Flies expressing Vap33^GD3990 under the control of Scer\GAL4^GMR.PF show a significant decrease in electroretinogram peak amplitude, despite showing normal eye development and largely undisturbed photoreceptor ultrastructure, as compared to controls.

(Yadav et al., 2018)

Pan-neuronal expression of Vap-33-1^GD3990 under the control of Scer\GAL4^elav.PU results in a decrease in bouton number and a concomitant increase in their size. An increased number of boutons show microtubule disorganisation compared to controls.

Expression of Vap-33-1^GD3990 in muscle under the control of Scer\GAL4^C57 produces a complex phenotype affecting both the pre- and post-synaptic compartments. An aberrant synaptic morphology is seen, although the number of boutons is normal. Boutons are larger and exhibit a highly irregular shape with a number of spike-like protrusions emerging from their contours.

(Forrest et al., 2013)

Adults expressing Vap-33A^GD3990 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Adults expressing Vap-33A^GD3990 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 50-60% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 50-60% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3990

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, Vap33^GD3990 has abnormal neuroanatomy phenotype, non-enhanceable by Sac1^GD2238, Scer\GAL4^elav.PU

(Forrest et al., 2013)

NOT Enhancer of

Statement

Reference

Vap33^GD3990, Scer\GAL4^elav.PU is a non-enhancer of abnormal neuroanatomy phenotype of Sac1^GD2238, Scer\GAL4^elav.PU

(Forrest et al., 2013)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, Vap33^GD3990 has NMJ bouton phenotype, non-enhanceable by Sac1^GD2238, Scer\GAL4^elav.PU

(Forrest et al., 2013)

NOT Enhancer of

Statement

Reference

Vap33^GD3990, Scer\GAL4^elav.PU is a non-enhancer of NMJ bouton phenotype of Sac1^GD2238, Scer\GAL4^elav.PU

(Forrest et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The bouton phenotypes in larvae co-expressing Sac1^GD2238 and Vap-33-1^GD3990 under the control of Scer\GAL4^elav.PU are no more severe than those seen in either mutant alone.

(Forrest et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v30404

w¹¹¹⁸; P{GD3990}v30404

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3990

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Vap-33-1^GD3990

Vap-33A^GD3990

Vap33^GD3990

dVAP-A^RNAi

(Yadav et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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