FB2025_02 , released April 17, 2025
Allele: Dmel\Xbp1GD4745
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General Information
Symbol
Dmel\Xbp1GD4745
Species
D. melanogaster
Name
FlyBase ID
FBal0209008
Feature type
allele
Associated gene
Dmel\Xbp1
Associated Insertion(s)
Carried in Construct
P{GD4745}
Also Known As
UAS-Xbp1RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD4745}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD4745
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Alzheimer's disease
      modeled by Hsap\APP2xAPP.SP.UAS
      (Casas-Tinto et al., 2011)
      ameliorates  carcinoma
      modeled by scrib1
      (Zheng et al., 2023)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Xbp1GD4745 under the control of Scer\GAL4Su(H).GBE (but not under either Scer\GAL4ppl.PU or Scer\GAL4how-24B) and using tub-Gal80[ts] to limit the time of expression, increases cell proliferation in the adult gut.

      (Wang et al., 2015)

      The temporally regulated expression (by Gal80[ts]) of Xbp1GD4745, for 5 days under the control of Scer\GAL4NP0001, or for 6 days under the control of Scer\GAL4NP5130, under the combined control of Scer\GAL4NP5130 and Scer\GAL80Su(H).GBE or under the control of Scer\GAL4Su(H).GBE, results in a strong increase in the proportion of mitotic cells in adult midguts, as compared to controls; expression for 6 days under the control of Scer\GAL4NP5130 also results in an increase in the population of intestinal stem cells, assessed by immmuno-staining for Delta and esg, in adult midguts as compared to controls. Clonal expression in the adult midgut leads to a strong increase in the average clone cell number and in the proportion of mitotic cell per midgut at 6 days after clone induction (when expression is under the control of Scer\GAL4Act.PU) and leads to a strong increase in the proportion of mitotic cell per midgut at 4 and 7 days after clone induction (when expression is under the control of Scer\GAL4unspecified), as compared to control clones.

      (Wang et al., 2014)

      Flies expressing Xbp1GD4745 in the developing eye, under the control of Scer\GAL4GMR.PF, exhibit slightly irregular and depigmented eyes but display mainly normal retinae.

      (Casas-Tinto et al., 2011)

      Expression of Xbp1GD4745 using Scer\GAL4ap-md544 results in semi-lethality. Adult survivors have severely disrupted wings which appear as small amorphic inflated structures, accompanied by marked excess of bristles on the wing and scutum, while the scutellum is not affected.

      Expression of Xbp1GD4745 using Scer\GAL4pnr-MD237 results in excess bristles on the mid-line of the scutum while the wings are not affected. No extra bristles are observed on the scutellum; some flies even exhibit a reduced number of scutellar bristles.

      (Bronstein et al., 2010)

      Expression of Xbp1GD4745 under the control of Scer\GAL4elav-C155 slightly increases the level of apoptosis in the developing nervous system.

      (Loewen and Feany, 2010)

      Adults expressing Xbp1GD4745 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 80-90% of the Scer\GAL4pnr-MD237 expression domain.

      Expression under the control of Scer\GAL4pnr-MD237 results in external sensory organs consisting of empty or multiple sockets in 30-40% of the Scer\GAL4pnr-MD237 expression domain of the notum.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Xbp1GD4745/Scer\GAL4hh-Gal4 is a non-enhancer of neoplasia | larval stage phenotype of Alg3tid2/Alg3tid1

      (de Vreede et al., 2018)
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Xbp1GD4745/Scer\GAL4hh-Gal4 is a non-suppressor of neoplasia | larval stage phenotype of Alg3tid2/Alg3tid1

      (de Vreede et al., 2018)
      Other
      Phenotype Manifest In
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Statement
      Reference

      Xbp1GD4745/Scer\GAL4hh-Gal4 is a non-enhancer of wing disc phenotype of Alg3tid2/Alg3tid1

      (de Vreede et al., 2018)
      Suppressor of
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased cell proliferation in the adult gut characteristic for flies expressing Xbp1GD4745 under the control of Scer\GAL4Su(H).GBE (using tub-Gal80[ts] to limit the time of expression) is suppressed by co-expression of bskGD10555 and partially inhibited by co-expression of upd3HMS00646.

      (Wang et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference

      Flies expressing Xbp1GD4745 and Hsap\APP2xAPP.SP.Scer\UAS in the developing eye (under the control of Scer\GAL4GMR.PF) exhibit very small and depigmented eyes with extremely thin and disorganised retinae.

      (Casas-Tinto et al., 2011)

      Co-expression of Xbp1GD4745 with Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4elav-C155 significantly increases the level of cell death seen in the nervous system.

      (Loewen and Feany, 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Xbp1GD4745
      Xbp1RNAi15347
      (Wang et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (16)