UASt regulatory sequences drive expression of an inverted repeat.
The expression of YpeGD8633 under the control of Scer\GAL4pnr-MD237 results in darkened small patches along the midline of the scutellum and posterior scutum, ectopic scutellar bristles and missing ocellar/postvertical bristles.
The expression of YpeGD8633 under the control of Scer\GAL4Act5C.PI results in developmental delay, with individuals reaching adulthood roughly 3-4days later than control, having difficulty eclosing (breaking the operculum but then becoming stuck and dying as they crawled out of the pupal case); adults exhibit darkened broad but discrete patches of cuticle across the body, wings that are held out laterally and curved downward, and ectopic scutellar bristles.
Adults expressing YpeGD8633 under the control of Scer\GAL4nub-AC-62 exhibit wings that are held out and curved downward.
Adults expressing YpeGD8633 under the control of Scer\GAL4r4 leads to delayed development to adulthood by 2-3 days.
Adults expressing YippeeGD17271 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 40-50% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.