Flies expressing aretGD8699 under the control of Scer\GAL4Mef2.PR are viable but entirely flightless. The indirect flight muscle fibers of day 1 adults begin to thin and rupture close to their thoracic attachment sites. Additionally, the sarcomeres appear too short and are sometimes lost. Myofibrils are variable in diameter and often hollow, in contrast to dense, regular myofibrils in wild type. A few days after eclosion, generally all IFM fibers are ruptured and the myofibrils entirely lose their sarcomeric organisation. The sarcomeres are of comparable length to wild type at 48 hours after puparium formation (APF), but fail to grow and even shorten by 72 hours APF. The tubular leg muscles do not display any obvious phenotypes.
Adults expressing aretGD8699 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in missing adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in degenerate myofibrils in the adult indirect flight muscles.
Expression under the control of Scer\GAL4Mef2.PR results in missing sarcomeres in the adult indirect flight muscles.