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General Information
Symbol
Dmel\bru1GD8699
Species
D. melanogaster
Name
FlyBase ID
FBal0209071
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing aretGD8699 under the control of Scer\GAL4Mef2.PR are viable but entirely flightless. The indirect flight muscle fibers of day 1 adults begin to thin and rupture close to their thoracic attachment sites. Additionally, the sarcomeres appear too short and are sometimes lost. Myofibrils are variable in diameter and often hollow, in contrast to dense, regular myofibrils in wild type. A few days after eclosion, generally all IFM fibers are ruptured and the myofibrils entirely lose their sarcomeric organisation. The sarcomeres are of comparable length to wild type at 48 hours after puparium formation (APF), but fail to grow and even shorten by 72 hours APF. The tubular leg muscles do not display any obvious phenotypes.

Flies expressing aretGD8699 under the control of Scer\GAL4Act88F.PB have significant defects in flight ability compared to wild type.

Adults expressing aretGD8699 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4Mef2.PR results in degenerate myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4Mef2.PR results in missing sarcomeres in the adult indirect flight muscles.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Mhc10 completely suppresses the indirect flight muscle degeneration seen in newly eclosed flies expressing aretGD8699 under the control of Scer\GAL4Mef2.PR.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
aretGD8699
bru1GD8699
Name Synonyms
Secondary FlyBase IDs
    References (9)