FlyBase Allele Report: Dmel\aux[GD7187]

Expression of aux^GD7187 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4^ple.PU or Scer\GAL4^Ddc.PU leads to age-progressive decline of the adult locomotor (climbing) abilities as well as shorter adult lifespan compared to controls. Additionally, expression under either of these drivers also results in age-dependent loss of dopaminergic neurons from the PPM1/2 cluster in the brain of aged (33 days old) but not young (3 days old) adult flies; with the Scer\GAL4^ple.PU driver the flies also display a moderate decrease in the number of PPM3 neurons regardless of age and with the Scer\GAL4^Ddc.PU driver aged flies display loss of PPL1 neurons too. Expression driven by the Scer\GAL4^GMR58E02 driver has no evident effect on the number of dopaminergic neurons from the PAM cluster (regardless of age).

(Song et al., 2017)

Adults expressing aux^GD7187 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7187

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

aux^GD7187, Scer\GAL4^Ddc.PU is an enhancer of abnormal neuroanatomy | adult stage phenotype of Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^Ddc.PU

(Song et al., 2017)

aux^GD7187, Scer\GAL4^Ddc.PU is an enhancer of decreased cell number | adult stage phenotype of Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^Ddc.PU

(Song et al., 2017)

Suppressor of

Statement

Reference

aux^GD7187, Scer\GAL4^GMR.long is a suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

Other

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has abnormal neuroanatomy | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has decreased cell number | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has abnormal neuroanatomy | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has decreased cell number | adult stage phenotype

(Song et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^repo.PU, aux^GD7187 has autophagosome | increased number | adult stage phenotype, suppressible by Hsap\GAK^UAS.Tag:FLAG, Scer\GAL4^repo.PU

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has adult glial cell phenotype, suppressible by Hsap\GAK^UAS.Tag:FLAG, Scer\GAL4^repo.PU

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has autophagosome | increased number | adult stage phenotype, suppressible by Atg1^HMS06014, Scer\GAL4^repo.PU

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has adult glial cell phenotype, suppressible by Atg1^HMS06014, Scer\GAL4^repo.PU

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has omegasome | increased number | adult stage phenotype, suppressible by Atg1^HMS06014, Scer\GAL4^repo.PU

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has autolysosome | increased number | adult stage phenotype, suppressible by Scer\GAL4^repo.PU/Syx17^JF01937

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has autolysosome | increased number | adult stage phenotype, suppressible by Scer\GAL4^repo.PU/Vamp7^GL01524

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has adult glial cell phenotype, suppressible by Scer\GAL4^repo.PU/Syx17^JF01937

(Zhang et al., 2023)

Scer\GAL4^repo.PU, aux^GD7187 has adult glial cell phenotype, suppressible by Scer\GAL4^repo.PU/Vamp7^GL01524

(Zhang et al., 2023)

Enhancer of

Statement

Reference

aux^GD7187, Scer\GAL4^Ddc.PU is an enhancer of dopaminergic PPM1 neuron | adult stage phenotype of Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^Ddc.PU

(Song et al., 2017)

aux^GD7187, Scer\GAL4^Ddc.PU is an enhancer of dopaminergic PPM2 neuron | adult stage phenotype of Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^Ddc.PU

(Song et al., 2017)

Suppressor of

Statement

Reference

aux^GD7187, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

aux^GD7187, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

Other

Statement

Reference

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has dopaminergic PPM2 neuron | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has dopaminergic PPM1 neuron | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has dopaminergic PPM2 neuron | adult stage phenotype

(Song et al., 2017)

Hsap\SNCA^A30P.UAS.cUa, Scer\GAL4^ple.PU, aux^GD7187 has dopaminergic PPM1 neuron | adult stage phenotype

(Song et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The loss of dopaminergic PPM1/2 neurons observed even in very young adult flies expressing Hsap\SNCA^{A30P.Scer\UAS.cUa} under the control of Scer\GAL4^Ddc.PU is exacerbated further by co-expression of aux^GD7187 and the number of neurons is further decreased. Simultaneous expression of the two transgenes driven by Scer\GAL4^ple.PU also leads to early-onset loss of PPM1/2, which is otherwise not observed in young (3 or 10 days old) flies expressing either of the transgenes alone with this driver. This is not due to loss of these neurons during development as their number is comparable to controls in third instar larvae as well as 1 day post-eclosion adults co-expressing Hsap\SNCA^{A30P.Scer\UAS.cUa} and aux^GD7187 (with Scer\GAL4^ple.PU or Scer\GAL4^Ddc.PU).

Co-expression of Hsap\SNCA^Scer\UAS.cUa (but not Hsap\SNCA^{A53T.Scer\UAS.cUa}) with aux^GD7187 leads to significant decrease in the number of dopaminergic PPM1/2 neurons in the brains of young (3 and/or 10 days old) adult flies, which is not observed at this age when any of the transgenes is expressed alone.

(Song et al., 2017)

Complementation and Rescue Data

Rescued by

aux^GD7187 is rescued by Scer\GAL4^ple.PU/aux^FL.UAS.GFP

(Song et al., 2017)

aux^GD7187 is rescued by Scer\GAL4^Ddc.PU/aux^FL.UAS.GFP

(Song et al., 2017)

Partially rescued by

aux^GD7187 is partially rescued by aux^{ΔCBD.UAS.Tag:FLAG}/Scer\GAL4^repo.PU

(Zhang et al., 2023)

aux^GD7187 is partially rescued by Scer\GAL4^repo.PU/aux^{ΔJ.UAS.Tag:FLAG}

(Zhang et al., 2023)

aux^GD7187 is partially rescued by Scer\GAL4^repo.PU/aux^{ΔPTEN.UAS.Tag:FLAG}

(Zhang et al., 2023)

aux^GD7187 is partially rescued by Scer\GAL4^repo.PU/aux^{DNAJC6m.UAS.Tag:FLAG}

(Zhang et al., 2023)

aux^GD7187 is partially rescued by Scer\GAL4^repo.PU/aux^UAS.Tag:FLAG

(Zhang et al., 2023)

Not rescued by

aux^GD7187 is not rescued by aux^{ΔKinase.UAS.Tag:FLAG}/Scer\GAL4^repo.PU

(Zhang et al., 2023)

Comments

The age-progressive rapid deterioration in adult locomotion (climbing ability) as well as the decreased adult lifespan characteristic for flies expressing aux^GD7187 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4^ple.PU or Scer\GAL4^Ddc.PU can be rescued by co-expression of aux^{FL.Scer\UAS.T:Avic\GFP}.

(Song et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v16182

w¹¹¹⁸; P{GD7187}v16182

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts