Allele Dmel\baz^GD1384

General Information
Symbol	Dmel\baz^GD1384	Species	D. melanogaster
Name		FlyBase ID	FBal0209111
Feature type	allele	Associated gene	Dmel\baz

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..328
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD1384} (Dickson et al., 2007.7.18, Dietzl et al., 2007, McDonald et al., 2008, Ramachandran et al., 2009, Wirtz-Peitz et al., 2008, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD1384
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act5C.PU (Dietzl et al., 2007) neuroanatomy defective \| larval stage, with Scer\GAL4^C57 (Ramachandran et al., 2009) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	border follicle cell, with Scer\GAL4^c306 (McDonald et al., 2008) embryonic/larval neuromuscular junction, with Scer\GAL4^C57 (Ramachandran et al., 2009) macrochaeta, with Scer\GAL4^pnr-MD237 (Dietzl et al., 2007) NMJ bouton, with Scer\GAL4^C57 (Ramachandran et al., 2009) trichogen cell \| supernumerary, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Expression under the control of Scer\GAL4[pnr-MD237] results in loss of bristles on the notum in 50-60% of the Scer\GAL4[pnr-MD237] expression domain. Expression under the control of Scer\GAL4[pnr-MD237] results in duplication of bristle hairs on the notum in 0-10% or 10-20% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. (Mummery-Widmer et al., 2009) Expression of baz[GD1384] under the control of Scer\GAL4[C57] in the muscles results in a reduction of postsynaptic F-actin at the larval neuromuscular junction. In addition, synaptic boutons are significantly reduced in number and show a significant increase in average volume compared to wild type. (Ramachandran et al., 2009) Expression of baz[GD1384] under the control of Scer\GAL4[c306] results in mild border follicle cell detachment defects as well as border cell migration defects. (McDonald et al., 2008) Expression of baz^GD1384 under the control of Scer\GAL4^pnr-MD237, results in missing macrochaetae, although this phenotype is mild. (Dietzl et al., 2007)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1384
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference baz^GD1384/Scer\GAL4¹⁴⁰⁷ is a suppressor of neuroanatomy defective phenotype of l(2)gl¹/l(2)gl⁴ (Wirtz-Peitz et al., 2008)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^pnr-MD237, baz^GD1384 has macrochaeta phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^pnr-MD237 (Dietzl et al., 2007)
Suppressor of
Statement Reference baz^GD1384/Scer\GAL4¹⁴⁰⁷ is a suppressor of embryonic/larval brain \| larval stage phenotype of l(2)gl¹/l(2)gl⁴ (Wirtz-Peitz et al., 2008) baz^GD1384/Scer\GAL4¹⁴⁰⁷ is a suppressor of embryonic/larval neuroblast phenotype of l(2)gl¹/l(2)gl⁴ (Wirtz-Peitz et al., 2008)
Other
Statement Reference Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, baz^GD1384 has eye phenotype (Dietzl et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference The overproliferation of larval brain neuroblasts seen in l(2)gl[1]/l(2)gl[4] animals is completely suppressed by expression of baz[GD1384] under the control of Scer\GAL4[1407]. (Wirtz-Peitz et al., 2008) While expression of baz^GD1384 alone, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF, does not lead to a significant eye phenotype, coexpression of Dcr-2^Scer\UAS.cDa with this transgene results in flies that have rough or necrotic eyes. Coexpression of Dcr-2^Scer\UAS.cDa enhances the missing macrochaetae phenotype of Scer\GAL4^pnr-MD237>baz^GD1384 flies. (Dietzl et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v2914 w¹¹¹⁸; P{GD1384}v2914 v2915 w¹¹¹⁸; P{GD1384}v2915
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1384
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	baz^GD1384 baz^RNAi (Wirtz-Peitz et al., 2008)
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Ramachandran et al., 2009, Dev. Neurobiol. 69(9): 583--602 A critical step for postsynaptic F-actin organization: Regulation of Baz/Par-3 localization by aPKC and PTEN. [FBrf0208224] McDonald et al., 2008, Curr. Biol. 18(21): 1659--1667 PAR-1 Kinase Regulates Epithelial Detachment and Directional Protrusion of Migrating Border Cells. [FBrf0206227] Wirtz-Peitz et al., 2008, Cell 135(1): 161--173 Linking cell cycle to asymmetric division: Aurora-A phosphorylates the Par complex to regulate Numb localization. [FBrf0206088] Dietzl et al., 2007, Nature 448(7150): 151--156 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Wirtz-Peitz et al., 2008, Cell 135(1): Supplemental Data. [FBrf0211096]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\bazGD1384

Allele Dmel\baz^GD1384