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General Information
Symbol
Dmel\botvGD2083
Species
D. melanogaster
Name
FlyBase ID
FBal0209171
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-only expression of botvGD2083 under the combined control of Scer\GAL4esg.PU and Gal80[ts] (induced by temperature shift from 18[o]C to 29[o]C) leads to adult posterior midguts showing increased mitotic index (pH3 staining), and increased numbers of stem cells and enteroblasts (i.e. esg-, Dl- and GBE+Su(H)-positive cells), but unchanged numbers of enteroendocrine cells (Pros-positive cells), as compared to controls; some of the progenitor cells (esg-positive) mis-express the enterocyte marker PDM1, indicative of intestinal homeostasis loss; the size of the polyploid cells expressing botvGD2083 is smaller than fully differentiated enterocytes, indicating a defect in maturation.

Expression of botvGD2083 in epidermic cells under the control of Scer\GAL4hh-Gal4 results in severe reduction in the overall dendrite or terminal dendrite density as well as terminal dendrite number within the intersegmental area in the adjacent class IV dendritic arborizing neurons in third instar larvae.

Adults expressing botvGD2083 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 20-30% of the Scer\GAL4pnr-MD237 expression domain.

Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 50-60% of the Scer\GAL4pnr-MD237 expression domain.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of botvGD2083 enhances the eye phenotype seen when Hsap\INSC96Y.Scer\UAS is expressed under the control of Scer\GAL4GMR.PF.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
botvGD2083
botvRNAi
Name Synonyms
Secondary FlyBase IDs
    References (8)