Allele Dmel\bowl^GD1389

General Information
Symbol	Dmel\bowl^GD1389	Species	D. melanogaster
Name		FlyBase ID	FBal0209172
Feature type	allele	Associated gene	Dmel\bowl

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..295
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD1389} (Dickson et al., 2007.7.18, Dietzl et al., 2007, Greenberg and Hatini, 2009, Mummery-Widmer et al., 2009, Del Signore et al., 2012)
Associated Sequence Features	dsRNA-GD1389
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act5C.PU (Dietzl et al., 2007) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^klu-G410 (Del Signore et al., 2012) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	chaeta \| supernumerary, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) eye, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH (Dietzl et al., 2007) prescutum, with Scer\GAL4^klu-G410 (Del Signore et al., 2012) thorax, with Scer\GAL4^pnr-MD237 (Dietzl et al., 2007)
Detailed Description
	Statement Reference Expression of bowl[GD1389] under the control of Scer\GAL4[klu-G410] results in a reduction in the extent of the prescutum and eliminates lateral microchaetae in the ventral prescutum. (Del Signore et al., 2012) Expression under the control of Scer\GAL4[pnr-MD237] results in extra bristles on the notum in 0% or 10-20% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in planar polarity defects in the orientation of bristles of the notum in 20-30% of the Scer\GAL4[pnr-MD237] expression domain. (Mummery-Widmer et al., 2009) Expression of bowl^GD1389 under the control of Scer\GAL4^pnr-MD237 leads to a mild thorax closure phenotype. Expression of bowl^GD1389 under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF leads to a mild rough eye phenotype. (Dietzl et al., 2007)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1389
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, bowl^GD1389 has lethal phenotype (Dietzl et al., 2007)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, bowl^GD1389 has eye phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH (Dietzl et al., 2007) Scer\GAL4^pnr-MD237, bowl^GD1389 has thorax phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^pnr-MD237 (Dietzl et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Coexpression of Dcr-2^Scer\UAS.cDa with bowl^GD1389, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF enhances the bowl^GD1389 rough eye phenotype and results in lethality. Coexpression of Dcr-2^Scer\UAS.cDa with bowl^GD1389, under the control of Scer\GAL4^pnr-MD237, enhances the bowl^GD1389 thorax closure phenotype. (Dietzl et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
VDRC	v3774 w¹¹¹⁸; P{GD1389}v3774 v3775 w¹¹¹⁸; P{GD1389}v3775 v18428 w¹¹¹⁸; P{GD1389}v18428/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1389
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	bowl^GD1389
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Del Signore et al., 2012, Mech. Dev. 129(5-8): 147--161 odd-skipped genes and lines organize the notum anterior-posterior axis using autonomous and non-autonomous mechanisms. [FBrf0219018] Greenberg and Hatini, 2009, Dev. Biol. 330(1): 93--104 Essential roles for lines in mediating leg and antennal proximodistal patterning and generating a stable Notch signaling interface at segment borders. [FBrf0208043] Dietzl et al., 2007, Nature 448(7150): 151--156 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\bowlGD1389

Allele Dmel\bowl^GD1389