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General Information
Symbol
Dmel\crbGD14463
Species
D. melanogaster
Name
FlyBase ID
FBal0209289
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
crb RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of crbGD14463 under the control of Scer\GAL4nub-AC-62 results in a moderate wing overgrowth in adult flies: the wing area is significantly increased compared to controls.

Expression of crbGD14463 under the control of Scer\GAL4btl.PS at 25[o]C does not alter the morphology of transition zone tubes.

Expression of crbGD14463 (co-expressed with Dcr-2Scer\UAS.cDa) under the control of Scer\GAL4repo.PU has no detectable effect on glial cell number in the third instar larval eye disc.

Stage 17 embryos expressing crbGD14463 under the control of Scer\GAL4btl.PS show severe defects in the transition from luminal liquid-clearance to air-filled airways.

Adults expressing crbGD14463 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of crbGD14463 under the control of Scer\GAL4ey.PH leads to an increase in head and eye size compared with controls.

Expression of crbGD14463 under the control of Scer\GAL4en-e16E leads to the overgrowth of the posterior compartment of the wing.

Expression under the control of Scer\GAL4pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The wing undergrowth observed in flies expressing either exScer\UAS.P\T.T:Avic\GFP-EGFP or kibraScer\UAS.P\T.cYa under the control of Scer\GAL4nub-AC-62 (their wing area is significantly decreased compared to controls) is modified by co-expression of crbGD14463 (which on its own leads to wing overgrowth), but whereas the small wing size of exScer\UAS.P\T.T:Avic\GFP-EGFP-expressing flies is increased, in the case of kibraScer\UAS.P\T.cYa, the wing size is further reduced by co-expression of crbGD14463.

Expression of crbGD14463 under the control of Scer\GAL4btl.PS partially suppresses the tracheal morphogenesis defects seen in GckIIIwheezy-770 mutant third instar larvae. Transition zone dilation is completely suppressed in 61% of terminal cells and partially suppressed in a further 27%.

The failure of anterior follicle cells in homozygous Taoeta stage 9 follicle cell clones to stretch is not suppressed if the clones are also expressing crbGD14463 under the control of Scer\GAL4GR1.

Xenogenetic Interactions
Statement
Reference

Co-expression of crbGD14463 significantly rescues the eye neurodegeneration phenotype caused by expression of Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU. The increased cell death phenotype which is seen in the eye imaginal discs of animals expressing Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU is also significantly suppressed.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
crbGD14463
Name Synonyms
Secondary FlyBase IDs
    References (13)