FlyBase Allele Report: Dmel\dom[GD1420]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\dom^GD1420

General Information

Symbol

Dmel\dom^GD1420

Species

D. melanogaster

Name

FlyBase ID

FBal0209379

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

Transgenic product class

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of developmental disorder of mental health

CEA

(Stessman et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | spermatogenesis, with Scer\GAL4^VP16.bam

(Prozzillo et al., 2023)

abnormal neuroanatomy, with Scer\GAL4²²¹

(Iyer et al., 2013)

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

decreased size | adult stage, with Scer\GAL4^ey.PU

(Prozzillo et al., 2021)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die before end of larval stage, with Scer\GAL4^Tub.PU

(Prozzillo et al., 2021)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible | adult stage, with Scer\GAL4^ey.PU

(Prozzillo et al., 2021)

Phenotype Manifest In

abdominal anterior ventral multidendritic neuron vdaa, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

chromatin | spermatogenesis, with Scer\GAL4^VP16.bam

(Prozzillo et al., 2023)

embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4^Tub.PU

(Prozzillo et al., 2021)

eye, with Scer\GAL4^ey.PU

(Prozzillo et al., 2021)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

larval dorsal multidendritic neuron ddaD, with Scer\GAL4²²¹

(Iyer et al., 2013)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval ventral multidendritic neuron vdaB, with Scer\GAL4^ppk.1.9

(Iyer et al., 2013)

meiotic spindle | spermatogenesis, with Scer\GAL4^VP16.bam

(Prozzillo et al., 2023)

meiotic spindle pole | increased number | spermatogenesis, with Scer\GAL4^VP16.bam

(Prozzillo et al., 2023)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

polytene chromosome | third instar larval stage, with Scer\GAL4^Tub.PU

(Prozzillo et al., 2021)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of dom^GD1420 under the control of Scer\GAL4^Tub.PU from larval hatching onwards leads to defects in third instar salivary gland polytene chromosomes (smaller and with a thin and disorganized structure).

(Prozzillo et al., 2021)

Expression of dom^GD1420 in C-I neurons under the control of Scer\GAL4²²¹ results in ddaD neurons that have an increased number of dendritic branches compared to controls. The total dendritic length is increased in ddaD neurons.

Expression of dom^GD1420 in C-IV neurons under the control of Scer\GAL4^ppk.1.9 results in a reduction in total dendritic length in ddaC, v'ada and vdaB neurons. The total number of dendritic branches is also reduced in ddaC neurons.

(Iyer et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain without any change in size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing dom^GD1420 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD1420} insertion line used.

Adults expressing dom^GD1420 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

Expression of dom^GD1420 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD1420} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1420

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

v7789

v7787

w¹¹¹⁸; P{GD1420}v7787

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1420

Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells

1420

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

dom^GD1420

(Bailetti et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections