Allele Dmel\ds^GD14350

General Information
Symbol	Dmel\ds^GD14350	Species	D. melanogaster
Name		FlyBase ID	FBal0209439
Feature type	allele	Associated gene	Dmel\ds

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..348
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD14350} (Mao et al., 2009, Dickson et al., 2007.7.18, Dietzl et al., 2007, Yue et al., 2012, Hogan et al., 2011, Olguín et al., 2011, Rauskolb et al., 2011, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD14350
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act5C.PU (Dietzl et al., 2007) planar polarity defective, with Scer\GAL4^Bx-MS1096 (Hogan et al., 2011) size defective, with Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^Bx-MS1096 (Hogan et al., 2011) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	crossvein, with Scer\GAL4^Bx-MS1096 (Hogan et al., 2011) eye, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH (Dietzl et al., 2007) mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) wing, with Scer\GAL4^Bx-MS1096 (Dietzl et al., 2007) wing, with Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011) wing blade, with Scer\GAL4^Bx-MS1096 (Hogan et al., 2011) wing disc \| posterior compartment, with Scer\GAL4^en-e16E (Mao et al., 2009)
Detailed Description
	Statement Reference Expression of ds[GD14350] under the control of Scer\GAL4[Bx-MS1096] in the dorsal region of the wing alters wing shape and disrupts crossveins, but produces only localised hair polarity changes in the proximal region. Expression of ds[GD14350] under the control of Scer\GAL4[Bx-MS1096] alters posterior ridges to a more anterior-posterior orientation, but does not affect anterior ridges. Expression of ds[GD14350] under the control of Scer\GAL4[salm-459.2] does not affect wing hair polarity. (Hogan et al., 2011) Expression of ds[GD14350] (together with Dcr-2[Scer\UAS.cDa]) using Scer\GAL4[nub-AC-62] results in overgrown wings. (Rauskolb et al., 2011) Expression of ds[GD14350] under the control of Scer\GAL4[en-e16E] results in a large increase in the relative size of the posterior compartment of the wing disc (where Scer\GAL4[en-e16E] is expressed) to 140% of anterior compartment size (in control wing discs, the posterior compartment is 80% of the size of the anterior compartment). (Mao et al., 2009) Expression under the control of Scer\GAL4[pnr-MD237] results in planar polarity defects in the orientation of bristles of the notum in 20-30% of the Scer\GAL4[pnr-MD237] expression domain. Expression under the control of Scer\GAL4[pnr-MD237] results in an enlarged notum. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 14350
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^nub-AC-62, ds^GD14350 has size defective phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011)
Other
Statement Reference Scer\GAL4^Bx-MS1096, ds^GD14350, pk³⁰ has planar polarity defective phenotype (Hogan et al., 2011) Scer\GAL4^Bx-MS1096, ds^GD14350, pk³⁰ has visible phenotype (Hogan et al., 2011) Scer\GAL4^Bx-MS1096, Scer\GAL80^ts.αTub84B, ds^GD14350, pk³⁰ has planar polarity defective phenotype (Hogan et al., 2011) Scer\GAL4^Bx-MS1096, Scer\GAL80^ts.αTub84B, ds^GD14350, pk³⁰ has visible phenotype (Hogan et al., 2011) Scer\GAL4^salm-459.2, ds^GD14350, pk³⁰ has planar polarity defective phenotype (Hogan et al., 2011) Scer\GAL4^salm-459.2, ds^GD14350, pk³⁰ has visible phenotype (Hogan et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^nub-AC-62, ds^GD14350 has wing phenotype, suppressible by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62 (Rauskolb et al., 2011)
Other
Statement Reference Scer\GAL4^Bx-MS1096, ds^GD14350, pk³⁰ has wing hair phenotype (Hogan et al., 2011) Scer\GAL4^Bx-MS1096, Scer\GAL80^ts.αTub84B, ds^GD14350, pk³⁰ has wing hair phenotype (Hogan et al., 2011) Scer\GAL4^salm-459.2, ds^GD14350, pk³⁰ has wing hair phenotype (Hogan et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference Expression of ds[GD14350] under the control of Scer\GAL4[Bx-MS1096] in the dorsal region of pk[30]-mutant wing modifies the pk[30] hair phenotype to a more distal polarity in the anterior and distal posterior regions. Expression of ds[GD14350] under the control of Scer\GAL4[salm-459.2] in a pk[30] mutant wings results in changes in wing hair polarity (relative to the wild-type pattern) outside of the main Scer\GAL4[salm-459.2] expression domain. When flies of the genotype Scer\GAL4[Bx-MS1096]/+; pk[30], ds[GD14350]/pk[30], Scer\GAL80[ts.αTub84B] are cultivated continuously at 18[o]C, they show a typical pk[30] mutant wing phenotype. When flies of the genotype Scer\GAL4[Bx-MS1096]/+; pk[30], ds[GD14350]/pk[30], Scer\GAL80[ts.αTub84B] are cultivated continuously at 30[o]C, they display wing morphology typical of reduced ds activity combined with more distal wing hair polarity than a pk[30] mutant. When flies of the genotype Scer\GAL4[Bx-MS1096]/+; pk[30], ds[GD14350]/pk[30], Scer\GAL80[ts.αTub84B] are shifted from 18[o]C to 30[o]C during pupal development, they show close to wild-type wing morphology, but a wing hair phenotype that is dependent upon timing of the temperature shift. Flies shifted before 30 hours after puparium formation (a.p.f), display the more distal wing hair polarity typical of continuous ds knockdown and a significant modification of the pk[30] wing hair phenotype is observed when pupae are shifted 36 hours a.p.f. However, pupae shifted after 40 hors a.p.f. display wing hair polarity phenotypes within the range of pk[30] mutant wings. (Hogan et al., 2011) The wing overgrowth phenotype associated with Scer\GAL4[nub-AC-62]-mediated expression of ds[GD14350] (together with Dcr-2[Scer\UAS.cDa]) is suppressed by co-expression of Zyx102EF[NIG.32018R]. (Rauskolb et al., 2011) Co-expression of lft[NIG.13139R] enhances the overgrowth of the the posterior compartment of the wing disc that is seen in animals expressing ds[GD14350] under the control of Scer\GAL4[en-e16E], such that the posterior compartment is 178% of anterior compartment size (in control wing discs, the posterior compartment is 80% of the size of the anterior compartment). (Mao et al., 2009)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
VDRC	v36219 w¹¹¹⁸; P{GD14350}v36219
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 14350
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ds^GD14350
Name Synonym
Secondary FlyBase IDs

References ( 8 )
Research paper	Yue et al., 2012, Dev. Cell 22(2): 255--267 The cell adhesion molecule echinoid functions as a tumor suppressor and upstream regulator of the hippo signaling pathway. [FBrf0217474] Hogan et al., 2011, PLoS Genet. 7(2): e1001305 Two frizzled planar cell polarity signals in the Drosophila wing are differentially organized by the fat/dachsous pathway. [FBrf0213159] Rauskolb et al., 2011, PLoS Biol. 9(6): e1000624 Zyxin links fat signaling to the hippo pathway. [FBrf0213900] Mao et al., 2009, Development 136(19): 3223--3233 Drosophila lowfat, a novel modulator of Fat signaling. [FBrf0209094] Dietzl et al., 2007, Nature 448(7150): 151--156 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Supplementary material	Olguín et al., 2011, Curr. Biol. 21(3): Supplemental Information. [FBrf0213857] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\dsGD14350

Allele Dmel\ds^GD14350