FlyBase Allele Report: Dmel\gish[GD10588]

General Information

Symbol

Dmel\gish^GD10588

Species

D. melanogaster

Name

FlyBase ID

FBal0209638

Feature type

allele

Associated gene

Dmel\gish

Associated Insertion(s)

Carried in Construct

P{GD10588}

Also Known As

UAS-gish^IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10588}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10588

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Howlett et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Weber et al., 2012)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

mature eight-cell ommatidial precursor cluster, with Scer\GAL4^sev.PU

(Gault et al., 2012)

ommatidium, with Scer\GAL4^hs.2sev

(Weber et al., 2012)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichome | increased number, with Scer\GAL4^pnr-MD237

(Gault et al., 2012)

wing | pupal stage | somatic clone, with Scer\GAL4^Act5C.PI

(Gault et al., 2012)

wing blade | pupal stage | somatic clone, with Scer\GAL4^Act5C.PI

(Gault et al., 2012)

wing hair | increased number, with Scer\GAL4^dpp.blk1

(Weber et al., 2012)

wing hair | increased number, with Scer\GAL4^en-e16E

(Gault et al., 2012, Weber et al., 2012)

Detailed Description

Statement

Reference

Wings expressing gish^GD10588 under the control of Scer\GAL4^en-e16E display trichome defects.

Expression of gish^GD10588 under the control of Scer\GAL4^pnr-MD237 in the thorax induces most of the cells to project multiple trichomes in contrast to the single posterior-oriented trichomes seen in wild-type.

Clones of cells expressing gish^GD10588 under the control of Scer\GAL4^sev.PU induce abnormally rotated ommatidial clusters.

(Gault et al., 2012)

Expression of gish^GD10588 under the control of Scer\GAL4^dpp.blk1 results in multiple cellular hairs in the wing.

Expression of gish^GD10588 under the control of Scer\GAL4^en-e16E results in multiple cellular hairs in the wing and defects in ommatidial rotation.

(Weber et al., 2012)

Adults expressing gish^GD10588 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing gish^GD10588 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10588

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, gish^GD10588 has abnormal planar polarity phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^hs.2sev

(Weber et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, gish^GD10588 has abnormal planar polarity phenotype, suppressible by nmo^DB/nmo[+]

(Weber et al., 2012)

Enhancer of

Statement

Reference

gish^GD10588/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

Scer\GAL4^hs.2sev/gish^GD10588 is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

Scer\GAL4^hs.2sev/gish^GD10588 is an enhancer of abnormal planar polarity phenotype of stan^sev.PD

(Weber et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/gish^GD10588 is a suppressor of bang sensitive | semidominant phenotype of para^bss1

(Howlett et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/gish^GD10588 is a non-suppressor of bang sensitive phenotype of para^bss1

(Howlett et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, gish^GD10588 has ommatidium phenotype, enhanceable by Dcr-2^UAS.cDa, Scer\GAL4^hs.2sev

(Weber et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, gish^GD10588 has wing hair | increased number phenotype, suppressible by Scer\GAL4^en-e16E/mwh^UAS.cYa

(Gault et al., 2012)

Scer\GAL4^hs.2sev, gish^GD10588 has ommatidium phenotype, suppressible by nmo^DB/nmo[+]

(Weber et al., 2012)

Enhancer of

Statement

Reference

gish^GD10588/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, smo^PKA12.UAS

(Li et al., 2016)

Scer\GAL4^en-e16E/gish^GD10588 is an enhancer of wing hair | increased number phenotype of mwh¹

(Gault et al., 2012)

gish^GD10588, Scer\GAL4^en-e16E is an enhancer of wing hair | increased number phenotype of Rab11^{Q70L.UASp.YFP}, Scer\GAL4^en-e16E

(Gault et al., 2012)

Scer\GAL4^hs.2sev/gish^GD10588 is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, dgo^UAS.cFa

(Weber et al., 2012)

Scer\GAL4^hs.2sev/gish^GD10588 is an enhancer of ommatidium phenotype of stan^sev.PD

(Weber et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of gish^GD10588 enhances the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096.

(Li et al., 2016)

Expression of gish^GD10588 under the control of Scer\GAL4^elav-C155 partially suppresses the bang sensitive phenotype seen in para^bss1/+ mutant females. Fewer flies exhibit bang-sensitivity and the threshold for seizure-like activity is partially rescued. gish^GD10588 does not suppress the bang sensitive phenotypes in para^bss1/para^bss1 or para^bss1/Y mutant flies.

(Howlett et al., 2013)

Expression of gish^GD10588 via Scer\GAL4^en-e16E strongly enhances the wing hair phenotype associated with homozygous mwh¹.

Co-expression of mwh^Scer\UAS.cYa with gish^GD10588 under the control of Scer\GAL4^en-e16E strongly suppresses the gish^GD10588-induced wing hair phenotype.

Co-expression of Rab11^{Q70L.Scer\UAS.P\T.T:Avic\GFP-YFP} and gish^GD10588 via Scer\GAL4^en-e16E results in a strong enhancement of the wing hair phenotype compared with that resulting from the expression of either transgene alone.

(Gault et al., 2012)

Co-expression of gish^GD10588 and Dcr-2^Scer\UAS.cDa using Scer\GAL4^hs.2sev results in planar cell polarity (PCP) and photoreceptor specification defects in ommatidia. The observed phenotypes include rare symmetrical ommatidia and R-cell loss.

Co-expression of gish^GD10588 with stan^sev.PD under the control of Scer\GAL4^hs.2sev enhances the ommatidial rotation defects induced by stan^sev.PD.

Co-expression of gish^GD10588 with fz^Scer\UAS.cAa under the control of Scer\GAL4^hs.2sev enhances the ommatidial rotation defects induced by Scer\GAL4^hs.2sev>fz^Scer\UAS.cAa.

nmo^DB/+ enhances the ommatidial rotation defects induced by Scer\GAL4^hs.2sev>gish^GD10588.

(Weber et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Co-expression of gish^{Scer\UAS.RB.T:Hsap\MYC} with gish^GD10588 under the control of Scer\GAL4^en-e16E partially rescues the trichome phenotype.

Co-expression of gish^{D187N.Scer\UAS.T:Hsap\MYC} with gish^GD10588 under the control of Scer\GAL4^en-e16E fails to rescue the trichome phenotype.

Co-expression of gish^{Scer\UAS.RF.T:Hsap\MYC} with gish^GD10588 under the control of Scer\GAL4^en-e16E fails to rescue the trichome phenotype.

An increase in apical cell area is observed in clones of Scer\GAL4^Act5C.PI-driven gish^GD10588-expressing cells in the pupal wing. Measurement of the apical area reveal a 60% increase relative to the adjacent wild-type cells, whereas analysis of the basolateral area does not show a significant increase. Transmission electron-microscopy reveal an increase in apical membrane projections in the mutant tissue compared with wild-type. Cells expressing gish^GD10588 via Scer\GAL4^Act5C.PI show no observable change in intensity or area of nuclear staining compared with wild-type cells.

(Gault et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v26003

w¹¹¹⁸; P{GD10588}v26003

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts