FlyBase Allele Report: Dmel\hep[GD1461]

General Information

Symbol

Dmel\hep^GD1461

Species

D. melanogaster

Name

FlyBase ID

FBal0209683

Feature type

allele

Associated gene

Dmel\hep

Associated Insertion(s)

Carried in Construct

P{GD1461}

Also Known As

hep RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1461}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1461

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | larval stage, with Scer\GAL4^He.PZ, Scer\GAL4^Hml.Δ

(Järvelä-Stölting et al., 2021)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval hemocyte | decreased number | larval stage, with Scer\GAL4^He.PZ, Scer\GAL4^Hml.Δ

(Järvelä-Stölting et al., 2021)

Detailed Description

Statement

Reference

Expression of hep^GD1461 in the posterior compartment of the imaginal wing disc, under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU does not affect levels of cell death.

(Wong et al., 2014)

Adults expressing hep^GD1461 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of hep^GD1461 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD1461} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1461

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

hep^GD1461, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Suppressor of

Statement

Reference

hep^GD1461, Scer\GAL4^cv-c-C5 is a suppressor of abnormal size | adult stage phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

hep^GD1461, Scer\GAL4^cv-c-C5 is a suppressor of visible | adult stage phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

hep^GD1461, Scer\GAL4^en.PU is a suppressor of increased cell death | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Hml.Δ, hep^GD1461, Scer\GAL4^He.PZ is a non-suppressor of increased cell number | larval stage phenotype of Prosα6^GD11466, Scer\GAL4^He.PZ, Scer\GAL4^Hml.Δ

(Järvelä-Stölting et al., 2021)

hep^GD1461, Scer\GAL4^en.PU is a non-suppressor of increased occurrence of cell division | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

Other

Statement

Reference

Mkk4^GD13804, Scer\GAL4^UAS.cNa, hep^GD1461 has abnormal stress response | somatic clone | adult stage phenotype

(Hsu et al., 2021)

Mkk4^KK101135, Scer\GAL4^UAS.cNa, hep^GD1461 has abnormal stress response | somatic clone | adult stage phenotype

(Hsu et al., 2021)

Scer\GAL4^hh.PU, hep^GD1461, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, hep^GD1461, mad2^GD16427 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

hep^GD1461/Scer\GAL4^{kn-col85-GAL4} is a non-enhancer of lamellocyte | larval stage phenotype of Scer\GAL4^{kn-col85-GAL4}, heca^GD8578

(Varga et al., 2019)

hep^GD1461, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

hep^GD1461, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

hep^GD1461, Scer\GAL4^GMR.PU is a non-enhancer of ommatidium phenotype of Hsap\LRRK2^UAS.cCa, Scer\GAL4^GMR.PU, grim^GMR.PU

(Chuang et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^Hml.Δ, hep^GD1461, Scer\GAL4^He.PZ is a suppressor | partially of lamellocyte | increased number | larval stage phenotype of Prosα6^GD11466, Scer\GAL4^He.PZ, Scer\GAL4^Hml.Δ

(Järvelä-Stölting et al., 2021)

hep^GD1461, Scer\GAL4^cv-c-C5 is a suppressor of wing phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

hep^GD1461, Scer\GAL4^en.PU is a suppressor of wing disc | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

hep^GD1461, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Hml.Δ, hep^GD1461, Scer\GAL4^He.PZ is a non-suppressor of embryonic/larval hemocyte | increased number | larval stage phenotype of Prosα6^GD11466, Scer\GAL4^He.PZ, Scer\GAL4^Hml.Δ

(Järvelä-Stölting et al., 2021)

hep^GD1461/Scer\GAL4^{kn-col85-GAL4} is a non-suppressor of lamellocyte | larval stage phenotype of Scer\GAL4^{kn-col85-GAL4}, heca^GD8578

(Varga et al., 2019)

hep^GD1461, Scer\GAL4^en.PU is a non-suppressor of wing disc | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

hep^GD1461, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

Other

Statement

Reference

Mkk4^GD13804, Scer\GAL4^UAS.cNa, hep^GD1461 has axon | adult stage phenotype

(Hsu et al., 2021)

Mkk4^GD13804, Scer\GAL4^UAS.cNa, hep^GD1461 has wing nerve | somatic clone phenotype

(Hsu et al., 2021)

Mkk4^KK101135, Scer\GAL4^UAS.cNa, hep^GD1461 has axon | adult stage phenotype

(Hsu et al., 2021)

Mkk4^KK101135, Scer\GAL4^UAS.cNa, hep^GD1461 has wing nerve | somatic clone phenotype

(Hsu et al., 2021)

Scer\GAL4^hh.PU, hep^GD1461, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, hep^GD1461, mad2^GD16427 has wing disc phenotype

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The small wing size induced by Scer\GAL4^C5-controlled expression of Dad1^GD1006 is suppressed by the co-expression of hep^GD1461.

Co-expression of hep^GD1461 suppresses the apoptosis, but not the compensatory cell division, induced by Scer\GAL4^en.PU-controlled expression of Dad1^GD1006 in third instar larval wing discs.

(Zhang et al., 2016)

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when hep^GD1461 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. This leads to an increase in cell death.

(Wong et al., 2014)

Xenogenetic Interactions

Statement

Reference

Co-expression of hep^GD1461 does not reverse the suppression of the grim^GMR.PU eye phenotype seen when Hsap\LRRK2^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

(Chuang et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v2968

w¹¹¹⁸ P{GD1461}v2968

v47507

w¹¹¹⁸; P{GD1461}v47507