Expression of Inx5^GD1177 under the control of Scer\GAL4^ey-OK107 in adults significantly reduces 3 hour aversive olfactory memory and the residual anaesthesia-resistant memory (ARM) after cold-shock, compared to controls; there is no effect on short-term memory or the anaesthesia-sensitive component of 3 hour memory, compared to controls. Expression under the control of Scer\GAL4^c739 or Scer\GAL4^VT049246, but not Scer\GAL4^VT030604, Scer\GAL4^VT044966 or Scer\GAL4^VT000765, also significantly reduces 3 hour aversive olfactory ARM, compared to controls; the effect seen with Scer\GAL4^c739 is lost when expression is restricted to a smaller subset of cells using Scer\GAL80^Mef2.mb247; the effect seen with Scer\GAL4^VT049246 is still seen when a Gal80[ts] transgene is used to restrict expression to the adult stage.

Expression of Inx5^GD1177 under the control of Scer\GAL4^ey-OK107, Scer\GAL4^VT049246, or Scer\GAL4^c739 does not cause any obvious difference in the gross morphology of the mushroom body, compared to controls.

Expression of inx5^GD1177 in both the anterior paired lateral (APL) and dorsal paired medial (DPM) neurons in the mushroom body (using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously, and in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) has no effect on 3 hour memory after associative conditioning.

Adults expressing Inx5^GD1177 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.