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General Information
Symbol
Dmel\Inx6GD3692
Species
D. melanogaster
Name
FlyBase ID
FBal0209738
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
inx6 RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Inx6GD3692 under the control of Scer\GAL4GMR23E10 in adults significantly decreases daytime and nighttime sleep duration, and sleep intensity, and significantly increases responsiveness to disturbance, compared to controls; when expression is restricted, using a Gal80[ts] transgene, to the 24 h prior to 48 h testing, daytime, but not nighttime, responsiveness is significantly increased, and daytime, but not nighttime, sleep intensity is significantly decreased, with no effect on sleep duration, compared to controls.

Expression of Inx6GD3692 under the control of Scer\GAL417d, the split-GAL4 pair of Scer\GAL4DBD.R41C07 and Hsap\RELAAD.R13F02, the split-GAL4 pair of Scer\GAL4DBD.R14C08 and Hsap\RELAAD.R12C11, or Scer\GAL4Mef2.247, but not the split-GAL4 pair of Scer\GAL4DBD.R89B01 and Hsap\RELAAD.R13F02, significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls; this effect is also seen when Inx6GD3692 is expressed during the adult stage only, under the control of Scer\GAL4Mef2.247.Switch (induced by RU486 application for three days prior to the experiment); no memory defect is seen using Scer\GAL417d when expression is restricted to a smaller subset of cells using Scer\GAL80Mef2.mb247.

Expression of inx6GD3692 in both the anterior paired lateral (APL) and dorsal paired medial (DPM) neurons in the mushroom body (using the Scer\GAL4GH146 and Scer\GAL4c316 drivers simultaneously, and in the presence of Dcr-2Scer\UAS.cDa to increase RNAi efficiency) results in impaired 3 hour (intermediate-term) memory after associative conditioning compared to controls.

Adults expressing Inx6GD3692 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Inx6GD3692 under the control of Scer\GAL4GMR23E10 blocks the secondary spikes and local field potential oscillations, but not the increased membrane potential, induced by acute optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with (red) light) of Scer\GAL4GMR23E10 cells in adults; this expression also suppresses the sleep duration and sleep intensity increases and the responsiveness decrease caused by prolonged optogenetic activation.

Prolonged optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with (red) light) of Scer\GAL4GMR23E10 cells in adults suppresses the sleep duration decrease, but not the responsiveness increase or the sleep intensity decrease caused by expression of Inx6GD3692 under the control of Scer\GAL4GMR23E10.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Inx6GD3692
inx6GD3692
Name Synonyms
Secondary FlyBase IDs
    References (9)