Clones in the salivary gland expressing Ns3^GD8441 under the control of Scer\GAL4^Act5C.PP show reduced cell size compared to control cells.

Adults expressing Ns3^GD8441 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Ns3^GD8441 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD8441} insertion line used.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with reduced Z-lines in the larval body wall muscles.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 80-90% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

Animals expressing ns3^GD8441 under the control of Scer\GAL4^Ddc.PL show a developmental delay and are reduced in weight compared to controls.