FlyBase Allele Report: Dmel\lin-52[GD12885]

General Information

Symbol

Dmel\lin-52^GD12885

Species

D. melanogaster

Name

FlyBase ID

FBal0209933

Feature type

allele

Associated gene

Dmel\lin-52

Associated Insertion(s)

Carried in Construct

P{GD12885}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12885}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12885

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

ommatidium, with Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Detailed Description

Statement

Reference

Expression of lin-52^GD12885 in larval eye-antennal discs, under the control of Scer\GAL4^GMR.PF, results in increased disorganisation of the ommatidia, specifically on the anterior part of the eye, which is characterised by fused ommatidia and loss of mechanosensory bristles. The degree of disorganisation is dose dependent and is enhanced when two copies of lin-52^GD12885 are present.

There is a slight increase in the level of cell proliferation in eye-antennal discs downregulating lin-52, through expression of lin-52^GD12885 under the control of Scer\GAL4^GMR.PF. However, there is a marked increase in the number of apoptotic cells at lower lin-52 levels.

lin-52^GD12885 eye disc clones induce enhanced hid expression and a patch of rough eye phenotype surrounded by normal ommatidia, suggesting regulation of hid by lin-52 is cell autonomous.

(Bhaskar et al., 2014)

Males expressing lin-52^GD12885 specifically in late spermatogonia and early spermatocytes using Scer\GAL4^{bam.T:Hsim\VP16} are fully fertile and display morphologically normal testes.

(Doggett et al., 2011)

Adults expressing lin-52^GD12885 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12885

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, enhanceable by Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, enhanceable by Avic\GFP^{EGFP.hid.5'F-E2FMut.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, enhanceable by Avic\GFP^{EGFP.hid.5'F-WT.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, non-enhanceable by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, non-enhanceable by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, suppressible by Df(3L)H99, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, suppressible by hid⁰⁵⁰¹⁴/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, non-suppressible by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype, non-suppressible by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Other

Statement

Reference

HDAC1^TRiP.cUa, Scer\GAL4^GMR.PF, lin-52^GD12885 has increased cell death phenotype

(Bhaskar et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, enhanceable by Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, enhanceable by Avic\GFP^{EGFP.hid.5'F-WT.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, enhanceable by Avic\GFP^{EGFP.hid.5'F-WT.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, enhanceable by Avic\GFP^{EGFP.hid.5'F-WT.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, enhanceable by Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, enhanceable by Rbf^120a/Rbf[+], Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, non-enhanceable by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, non-enhanceable by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, non-enhanceable by Avic\GFP^{EGFP.hid.5'F-E2FMut.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, non-enhanceable by Avic\GFP^{EGFP.hid.5'F-WT.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, non-enhanceable by Avic\GFP^{EGFP.hid.5'F-WT.Tag:NLS(SV40-largeT)}, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, non-enhanceable by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, non-enhanceable by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, non-enhanceable by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, non-enhanceable by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, suppressible by hid⁰⁵⁰¹⁴/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, suppressible by hid⁰⁵⁰¹⁴/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, suppressible by hid⁰⁵⁰¹⁴/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, suppressible by Df(3L)H99, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, suppressible by Df(3L)H99, Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, non-suppressible by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, non-suppressible by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, non-suppressible by rpr⁸⁷/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype, non-suppressible by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype, non-suppressible by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype, non-suppressible by grim^MB10379/Scer\GAL4^GMR.PF

(Bhaskar et al., 2014)

Other

Statement

Reference

HDAC1^TRiP.cUa, Scer\GAL4^GMR.PF, lin-52^GD12885 has eye phenotype

(Bhaskar et al., 2014)

HDAC1^TRiP.cUa, Scer\GAL4^GMR.PF, lin-52^GD12885 has ommatidium phenotype

(Bhaskar et al., 2014)

HDAC1^TRiP.cUa, Scer\GAL4^GMR.PF, lin-52^GD12885 has interommatidial bristle phenotype

(Bhaskar et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Downregulation of lin-52 in the eye-antennal disc in a apoptosis-negative environment, through expression of lin-52^GD12885 under the control of Scer\GAL4^GMR.PF in a Df(3L)H99 background, results in a suppression of the lin-52-associated eye phenotype.

Overexpression of Diap1^Scer\UAS.cUa in the eye-antennal disc expressing lin-52^GD12885, with both transgenes under the control of Scer\GAL4^GMR.PF, suppresses the increase in apoptosis seen upon downregulation of lin-52 alone.

A rpr⁸⁷ or grim^MB10379 mutant background does not affect the Scer\GAL4^GMR.PF>lin-52^GD12885 eye phenotype, and the number of dying cells remains unaltered.

A hid⁰⁵⁰¹⁴ mutant background suppresses the eye phenotype seen upon expression of lin-52^GD12885 under the control of Scer\GAL4^GMR.PF, along with a dramatic reduction in apoptosis in the eye-antennal disc.

A Rbf^120a mutant background dominantly enhances the Scer\GAL4^GMR.PF>lin-52^GD12885 eye phenotype. This is accompanied by a corresponding increase in hid protein and elevated apoptosis in the eye-antennal disc.

An E2f1^rM729 mutant background dominantly enhances the Scer\GAL4^GMR.PF>lin-52^GD12885 eye phenotype. This is accompanied by a corresponding increase in hid protein and elevated apoptosis in the eye-antennal disc.

An E2f2³²⁹ mutant background dominantly enhances the Scer\GAL4^GMR.PF>lin-52^GD12885 eye phenotype. This is accompanied by a corresponding increase in hid protein and elevated apoptosis in the eye-antennal disc.

Co-expression of Rpd3^TRiP.cUa and lin-52^GD12885 together in the developing eye, under the control of Scer\GAL4^GMR.PF, results in a spotted eye phenotype. These eye-antennal discs display activation of hid, in cells adjacent to the morphogenetic furrow and extending further up to the distal end of the eye-antennal discs.

(Bhaskar et al., 2014)

Xenogenetic Interactions

Statement

Reference

Co-expression of Avic\GFP^{EGFP.W.5'F-WT.T:SV40\nls-largeT} enhances the Scer\GAL4^GMR.PF>lin-52^GD12885 adult eye phenotype.

Co-expression of Avic\GFP^{EGFP.W.5'F-E2FMut.T:SV40\nls-largeT} fails to modify the Scer\GAL4^GMR.PF>lin-52^GD12885 adult eye phenotype.

(Bhaskar et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type