FlyBase Allele Report: Dmel\mei-41[GD4258]

General Information

Symbol

Dmel\mei-41^GD4258

Species

D. melanogaster

Name

FlyBase ID

FBal0210058

Feature type

allele

Associated gene

Dmel\mei-41

Associated Insertion(s)

Carried in Construct

P{GD4258}

Also Known As

UAS-mei-41^RNAi

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD4258}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mei-41

Associated Sequence Features

dsRNA-GD4258

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with BacA\p35^UAS.cHa

(Dekanty et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is ameliorated by bsk^K53R.UAS

(Dekanty et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal cell shape | adult stage, with Scer\GAL4^NP5130

(Park et al., 2015)

abnormal DNA repair | adult stage | conditional, with Scer\GAL4^NP5130

(Park et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^NP5130

(Park et al., 2015)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Park et al., 2015)

short lived, with Scer\GAL4^NP5130

(Park et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^NP5130

(Park et al., 2015)

enteroblast of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Park et al., 2015)

enteroblast of posterior adult midgut epithelium | conditional, with Scer\GAL4^NP5130

(Park et al., 2015)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Park et al., 2015)

intestinal stem cell of posterior adult midgut epithelium | conditional, with Scer\GAL4^NP5130

(Park et al., 2015)

Detailed Description

Statement

Reference

The adulthood-only expression of mei-41^GD4258 under the control of Scer\GAL4^esg-NP5130 (in combination with Gal80[ts], for the temporal control of expression) leads to a severe decrease in life-span, leads to a significant decrease in cell density in the adult midgut, leads to larger and rounder adult midgut progenitor cells (esg-positive intestinal stem cells and enteroblasts), and leads to the adult intestinal stem cells showing significant decreases in relative cell number and in the frequency of DNA replication, as compared to controls. Upon γ-radiation, the adult midgut progenitor cells show an increase in DNA damage, assessed by pS/TQ and γH2AvD immunofluorescence, as compared to irradiated controls.

(Park et al., 2015)

Adults expressing mei-41^GD4258 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4258

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, mei-41^GD4258 has hyperplasia | larval stage | conditional phenotype, suppressible by bsk^K53R.UAS, Scer\GAL4^en.PU

(Dekanty et al., 2015)

Enhancer of

Statement

Reference

mei-41^GD4258, Scer\GAL4^en.PU is an enhancer of hyperplasia | larval stage | conditional phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Dekanty et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, mei-41^GD4258 has wing disc posterior compartment | conditional phenotype, suppressible by bsk^K53R.UAS, Scer\GAL4^en.PU

(Dekanty et al., 2015)

Enhancer of

Statement

Reference

mei-41^GD4258, Scer\GAL4^en.PU is an enhancer of wing disc posterior compartment | conditional phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en.PU

(Dekanty et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of mei-41^GD4258 enhances the ionizing irradiation-induced enlarged wing disc posterior compartment observed in individuals expressing BacA\p35^UAS.cHa under the control of Scer\GAL4^en.PU; this phenotype is suppressed by the additional co-expression of bsk^K53R.UAS.

(Dekanty et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v11251

w¹¹¹⁸; P{GD4258}v11251

Notes on Origin

Discoverer