FlyBase Allele Report: Dmel\milt[GD8116]

General Information

Symbol

Dmel\milt^GD8116

Species

D. melanogaster

Name

FlyBase ID

FBal0210074

Feature type

allele

Associated gene

Dmel\milt

Associated Insertion(s)

Carried in Construct

P{GD8116}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8116}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8116

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Iijima-Ando et al., 2009)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Iijima-Ando et al., 2012)

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^elav-C155

(Iijima-Ando et al., 2009)

abnormal neuroanatomy | conditional, with Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of milt^GD8116 in eyes under the control of Scer\GAL4^GMR.PF results in loss of mitochondria in the synaptic terminals of photoreceptor neurons in 3 day old flies. In addition, the presynaptic terminals contain vesicles with a wider range of sizes than in controls. No axonal or presynaptic neurodegeneration is detected in the lamina of these flies at 3 day old, but degeneration is seen in the lamina at 20 days, and more strongly at 30 days. When milt^GD8116 is expressed under the control of both Scer\GAL4^GMR.PF and Scer\GAL4^elav-C155 neuronal degeneration is seen in 21 day-old flies, with the most prominent degeneration in the optic lobe. No degeneration is seen in the laminae of 30-day old wild type flies.

(Iijima-Ando et al., 2012)

Glial cells show mis-distribution of mitochondria in third instar larvae expressing milt^GD8116 under the simultaneous control of both Scer\GAL4^repo.PL and Scer\GAL4^repo.

(Schmidt et al., 2012)

Adults expressing milt^GD8116 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD8116} insertion line used.

(Neely et al., 2010)

Neuronal knockdown of milt by milt^GD8116 driven by Scer\GAL4^elav-C155 does not affect locomotor function up to 10 days after eclosion, but causes locomotor dysfunction after 17 days.

(Iijima-Ando et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8116

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF, milt^GD8116 has abnormal neuroanatomy | adult stage | conditional phenotype, suppressible by par-1^{RNAi.UAS.cMDa}, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Scer\GAL4^GMR.PF, milt^GD8116 has abnormal neuroanatomy | adult stage phenotype, suppressible by tau^HM05101/Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Scer\GAL4^GMR.PF, milt^GD8116 has abnormal neuroanatomy | adult stage phenotype, suppressible by par-1^{RNAi.UAS.cMDa}/Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Enhancer of

Statement

Reference

milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of abnormal neuroanatomy | adult stage | conditional phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

NOT Enhancer of

Statement

Reference

milt^GD8116, Scer\GAL4^GMR.PF is a non-enhancer of abnormal neuroanatomy | adult stage | conditional phenotype of Hsap\MAPT^{S262A.UAS.0N4R.cIAa}, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Other

Statement

Reference

Scer\GAL4^GMR.PF, milt^GD8116, par-1^UAS.Tag:MYC has abnormal neuroanatomy phenotype

(Iijima-Ando et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF, milt^GD8116 has lamina phenotype, suppressible by par-1^{RNAi.UAS.cMDa}, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Scer\GAL4^GMR.PF, milt^GD8116 has lamina phenotype, suppressible by tau^HM05101/Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Scer\GAL4^GMR.PF, milt^GD8116 has lamina phenotype, suppressible by par-1^{RNAi.UAS.cMDa}/Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, milt^GD8116 has photoreceptor neuron phenotype, non-suppressible by Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Scer\GAL4^GMR.PF, milt^GD8116 has mitochondrion phenotype, non-suppressible by Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Enhancer of

Statement

Reference

milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of lamina phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of autophagosome phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of multivesicular body phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

NOT Enhancer of

Statement

Reference

milt^GD8116, Scer\GAL4^GMR.PF is a non-enhancer of lamina phenotype of Hsap\MAPT^{S262A.UAS.0N4R.cIAa}, Scer\GAL4^GMR.PF

(Iijima-Ando et al., 2012)

Other

Statement

Reference

Scer\GAL4^GMR.PF, milt^GD8116, par-1^UAS.Tag:MYC has lamina phenotype

(Iijima-Ando et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of par-1^{Scer\UAS.T:Hsap\MYC} and milt^GD8116 under the control of Scer\GAL4^GMR.PF causes prominent neurodegeneration in the lamina at 3 days old.

Expression of tau^HM05101 suppresses the neurodegeneration seen in the lamina of 30 day old flies when milt^GD8116 is expressed under the control of Scer\GAL4^GMR.PF.

Expression of par-1^{dsRNA.Scer\UAS.cMDa} suppresses the neurodegeneration seen in the lamina of 30 day old flies when milt^GD8116 is expressed under the control of Scer\GAL4^GMR.PF.

(Iijima-Ando et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of milt^GD8116 dramatically enhances neurodegeneration in the lamina of 3 day old flies expressing Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PF and as in flies expressing milt^GD8116 alone, there is a reduction in the number of mitochondria in the synaptic terminals of photoreceptor neurons.

Expression of milt^GD8116 enhances the defects in axon pathology seen when Hsap\MAPT^Scer\UAS.cWa is expressed under the control of Scer\GAL4^GMR.PF. No neurofibrillary tangles seen in these flies. This enhancement is suppressed upon co-expression of par-1^{dsRNA.Scer\UAS.cMDa}.

Expression of milt^GD8116 does not enhance neurodegeneration in the lamina of 3 day old flies expressing Hsap\MAPT^{Scer\UAS.S262A} under the control of Scer\GAL4^GMR.PF.

(Iijima-Ando et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v41508

w¹¹¹⁸ P{GD8116}v41508

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8116

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

milt^GD8116

milt^dsRNA

(Schmidt et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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