Allele Dmel\milt^GD8116

General Information
Symbol	Dmel\milt^GD8116	Species	D. melanogaster
Name		FlyBase ID	FBal0210074
Feature type	allele	Associated gene	Dmel\milt

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..303
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD8116} (Dickson et al., 2007.7.18, Iijima-Ando et al., 2009, Mummery-Widmer et al., 2009, Iijima-Ando et al., 2012)
Associated Sequence Features	dsRNA-GD8116
Cytology
Phenotypic Data
Phenotypic Class
	locomotor behavior defective, with Scer\GAL4^elav-C155 (Iijima-Ando et al., 2009) neuroanatomy defective \| conditional, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	embryonic/larval glial cell, with Scer\GAL4^repo.PL, Scer\GAL4^repo (Schmidt et al., 2012) mitochondrion, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) mitochondrion, with Scer\GAL4^repo.PL, Scer\GAL4^repo (Schmidt et al., 2012) optic lobe \| conditional, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) photoreceptor cell, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) synaptic vesicle, with Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
Detailed Description
	Statement Reference Expression of milt[GD8116] in eyes under the control of Scer\GAL4[GMR.PF] results in loss of mitochondria in the synaptic terminals of photoreceptor neurons in 3 day old flies. In addition, the presynaptic terminals contain vesicles with a wider range of sizes than in controls. No axonal or presynaptic neurodegeneration is detected in the lamina of these flies at 3 day old, but degeneration is seen in the lamina at 20 days, and more strongly at 30 days. When milt[GD8116] is expressed under the control of both Scer\GAL4[GMR.PF] and Scer\GAL4[elav-C155] neuronal degeneration is seen in 21 day-old flies, with the most prominent degeneration in the optic lobe. No degeneration is seen in the laminae of 30-day old wild type flies. (Iijima-Ando et al., 2012) Glial cells show mis-distribution of mitochondria in third instar larvae expressing milt[GD8116] under the simultaneous control of both Scer\GAL4[repo.PL] and Scer\GAL4[repo]. (Schmidt et al., 2012) Neuronal knockdown of milt by milt[GD8116] driven by Scer\GAL4[elav-C155] does not affect locomotor function up to 10 days after eclosion, but causes locomotor dysfunction after 17 days. (Iijima-Ando et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 8116
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF, milt^GD8116 has neuroanatomy defective \| adult stage \| conditional phenotype, suppressible by par-1^{dsRNA.Scer\UAS.cMDa}, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) Scer\GAL4^GMR.PF, milt^GD8116 has neuroanatomy defective \| adult stage phenotype, suppressible by par-1^{dsRNA.Scer\UAS.cMDa}/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) Scer\GAL4^GMR.PF, milt^GD8116 has neuroanatomy defective \| adult stage phenotype, suppressible by tau^HM05101/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
Enhancer of
Statement Reference milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of neuroanatomy defective \| adult stage \| conditional phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
NOT Enhancer of
Statement Reference milt^GD8116, Scer\GAL4^GMR.PF is a non-enhancer of neuroanatomy defective \| adult stage \| conditional phenotype of Hsap\MAPT^{Scer\UAS.S262A}, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
Other
Statement Reference Scer\GAL4^GMR.PF, milt^GD8116, par-1^{Scer\UAS.T:Hsap\MYC} has neuroanatomy defective phenotype (Iijima-Ando et al., 2012)
Phenotype Manifest In
Suppressed by
Statement Reference Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF, milt^GD8116 has lamina phenotype, suppressible by par-1^{dsRNA.Scer\UAS.cMDa}, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) Scer\GAL4^GMR.PF, milt^GD8116 has lamina phenotype, suppressible by par-1^{dsRNA.Scer\UAS.cMDa}/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) Scer\GAL4^GMR.PF, milt^GD8116 has lamina phenotype, suppressible by tau^HM05101/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
NOT suppressed by
Statement Reference Scer\GAL4^GMR.PF, milt^GD8116 has mitochondrion phenotype, non-suppressible by Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) Scer\GAL4^GMR.PF, milt^GD8116 has photoreceptor cell phenotype, non-suppressible by Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
Enhancer of
Statement Reference milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of autophagic vacuole phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of lamina phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012) milt^GD8116, Scer\GAL4^GMR.PF is an enhancer of multivesicular body phenotype of Hsap\MAPT^Scer\UAS.cWa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
NOT Enhancer of
Statement Reference milt^GD8116, Scer\GAL4^GMR.PF is a non-enhancer of lamina phenotype of Hsap\MAPT^{Scer\UAS.S262A}, Scer\GAL4^GMR.PF (Iijima-Ando et al., 2012)
Other
Statement Reference Scer\GAL4^GMR.PF, milt^GD8116, par-1^{Scer\UAS.T:Hsap\MYC} has lamina phenotype (Iijima-Ando et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of par-1[Scer\UAS.T:Hsap\MYC] and milt[GD8116] under the control of Scer\GAL4[GMR.PF] causes prominent neurodegeneration in the lamina at 3 days old. Expression of tau[HM05101] suppresses the neurodegeneration seen in the lamina of 30 day old flies when milt[GD8116] is expressed under the control of Scer\GAL4[GMR.PF]. Expression of par-1[dsRNA.Scer\UAS.cMDa] suppresses the neurodegeneration seen in the lamina of 30 day old flies when milt[GD8116] is expressed under the control of Scer\GAL4[GMR.PF]. (Iijima-Ando et al., 2012)
Xenogenetic Interactions
Statement Reference Expression of milt[GD8116] dramatically enhances neurodegeneration in the lamina of 3 day old flies expressing Hsap\MAPT[Scer\UAS.cWa] under the control of Scer\GAL4[GMR.PF] and as in flies expressing milt[GD8116] alone, there is a reduction in the number of mitochondria in the synaptic terminals of photoreceptor neurons. Expression of milt[GD8116] enhances the defects in axon pathology seen when Hsap\MAPT[Scer\UAS.cWa] is expressed under the control of Scer\GAL4[GMR.PF]. No neurofibrillary tangles seen in these flies. This enhancement is suppressed upon co-expression of par-1[dsRNA.Scer\UAS.cMDa]. Expression of milt[GD8116] does not enhance neurodegeneration in the lamina of 3 day old flies expressing Hsap\MAPT[Scer\UAS.S262A] under the control of Scer\GAL4[GMR.PF]. (Iijima-Ando et al., 2012)
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v41508 w¹¹¹⁸ P{GD8116}v41508 v41507 w¹¹¹⁸; P{GD8116}v41507/TM3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 8116
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	milt^dsRNA (Schmidt et al., 2012) milt^GD8116
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Iijima-Ando et al., 2012, PLoS Genet. 8(8): e1002918 Loss of Axonal Mitochondria Promotes Tau-Mediated Neurodegeneration and Alzheimer's Disease-Related Tau Phosphorylation Via PAR-1. [FBrf0219369] Schmidt et al., 2012, J. Neurosci. 32(22): 7466--7476 Kinesin heavy chain function in Drosophila glial cells controls neuronal activity. [FBrf0218491] Iijima-Ando et al., 2009, PLoS ONE 4(12): e8310 Mitochondrial mislocalization underlies Abeta42-induced neuronal dysfunction in a Drosophila model of Alzheimer's disease. [FBrf0209556]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\miltGD8116

Allele Dmel\milt^GD8116