UASt regulatory sequences drive expression of an inverted repeat.
Expression of nmoGD1495 under the control of Scer\GAL4elav-C155 (co-expressed with Dcr-2Scer\UAS.cDa) has no effect on neuromuscular junction evoked excitatory postsynaptic potential (eEPSP) amplitude compared to controls.
Flies expressing nmoGD1495 under the control of Scer\GAL4tim.Scer\UAS manifest significantly shorter periods than do parental controls. nmoGD1495-expression results in free running periods of approximately 21-22 hours.
Adults expressing nmoGD1495 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.