FlyBase Allele Report: Dmel\park[GD5543]

General Information

Symbol

Dmel\park^GD5543

Species

D. melanogaster

Name

FlyBase ID

FBal0210298

Feature type

allele

Associated gene

Dmel\park

Associated Insertion(s)

Carried in Construct

P{GD5543}

Also Known As

UAS-Parkin-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5543}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5543

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Parkinson's disease

CEA

(Cornelissen et al., 2014)

model of Parkinson's disease 2

CEC

(Fevga et al., 2023)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Parkinson's disease

is ameliorated by Usp32^GD5871

(Cornelissen et al., 2014)

ameliorates amyotrophic lateral sclerosis type 6

modeled by Hsap\FUS^{WT.UAS.RFP(Unk),Tag:HA}

(Chen et al., 2016)

ameliorates frontotemporal dementia and/or amyotrophic lateral sclerosis 2

modeled by Chchd2^S81L.UAS

(Baek et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Venderova et al., 2009)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

mitochondrion, with Scer\GAL4^Mef2.PU

(Cornelissen et al., 2014)

mitochondrion, with Scer\GAL4^Tub.PU

(Cornelissen et al., 2014)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Scer\GAL4^Mef2.PU-mediated expression of park^GD5543 results in the appearance of swollen/clumped mitochondria in adult indirect flight muscles. EM analysis reveals vacuolization of adult flight muscle cells and severely swollen mitochondria with disrupted cristae. Mitochondrial membrane potential in Scer\GAL4^tub.PU park^GD5543 flies is reduced, and 2-week-old adult flies show a defect in climbing ability.

(Cornelissen et al., 2014)

Adults expressing park^GD5543 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of park^GD5543 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD5543} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies.

Expression under the control of Scer\GAL4^Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with broadened Z-lines in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

Flies expressing park^GD5543 under the control of Scer\GAL4^GMR.PF at 29[o]C show loss of pigmentation in the eyes.

(Venderova et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5543

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, park^GD5543 has abnormal locomotor behavior phenotype, suppressible by Scer\GAL4^Tub.PU/Usp32^GD5871

(Cornelissen et al., 2014)

Suppressor of

Statement

Reference

park^GD5543, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage | progressive phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Mef2.PU, park^GD5543 has mitochondrion phenotype, suppressible by Scer\GAL4^Mef2.PU/Usp32^GD5871

(Cornelissen et al., 2014)

Scer\GAL4^Tub.PU, park^GD5543 has mitochondrion phenotype, suppressible by Scer\GAL4^Tub.PU/Usp32^GD5871

(Cornelissen et al., 2014)

Suppressor of

Statement

Reference

park^GD5543, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of CG8334^GD5871 largely rescues the mitochondrial defects and defective climbing ability seen park^GD5543 Scer\GAL4^Mef2.PU or park^GD5543 Scer\GAL4^tub.PU flies.

(Cornelissen et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v47636