FlyBase Allele Report: Dmel\peb[GD1507]

General Information

Symbol

Dmel\peb^GD1507

Species

D. melanogaster

Name

FlyBase ID

FBal0210317

Feature type

allele

Associated gene

Dmel\peb

Associated Insertion(s)

Carried in Construct

P{GD1507}

Also Known As

UAS-hnt^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1507}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1507

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GMR.PU

(Oliva et al., 2015)

abnormal oviposition, with Scer\GAL4^GMR44E10

(Deady et al., 2017)

abnormal oviposition, with Scer\GAL4^GMR47A04

(Deady et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Females expressing peb^GD1507 under the control of Scer\GAL4^GMR44E10 or Scer\GAL4^GMR47A04 lay significantly fewer eggs, show significantly longer ovulation time despite of no changes in the time spent by eggs in the oviduct and uterus, and show a significant decrease in octopamine- or ionomycin-induced follicle rupture (ex vivo treated egg chambers), as compared to controls; no other obvious oogenesis defects are observed, as plenty of stage-14 egg chambers are produced.

Stage 14 egg chambers from females expressing peb^GD1507 under the control of Scer\GAL4^GMR47A04 show an intact or broken posterior basement membrane, instead of the mostly open posterior basement membrane observed in controls.

(Deady et al., 2017)

Expression of peb^GD1507 driven by Scer\GAL4^GMR.PU leads to significant mistargeting and overshooting of R1-R6 axons towards the medulla layers, compared to controls.

(Oliva et al., 2015)

Adults expressing peb^GD1507 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1507

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR44E10, peb^GD1507 has abnormal oviposition phenotype, enhanceable by Hsap\RREB1^UAS.EGFP, Scer\GAL4^GMR44E10

(Deady et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, peb^GD1507 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by jbug^UAS.L.Tag:HA/Scer\GAL4^GMR.PU

(Oliva et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR44E10, peb^GD1507 has egg chamber | oogenesis stage S14 phenotype, suppressible by Hsap\RREB1^UAS.EGFP, Scer\GAL4^GMR44E10

(Deady et al., 2017)

Scer\GAL4^GMR44E10, peb^GD1507 has oocyte associated follicular epithelium | oogenesis stage S14 phenotype, suppressible by Hsap\RREB1^UAS.EGFP, Scer\GAL4^GMR44E10

(Deady et al., 2017)

Scer\GAL4^GMR47A04, peb^GD1507 has egg chamber | oogenesis stage S14 phenotype, suppressible | partially by Oamb^UAS.K3, Scer\GAL4^GMR47A04

(Deady et al., 2017)

Scer\GAL4^GMR47A04, peb^GD1507 has oocyte associated follicular epithelium | oogenesis stage S14 phenotype, suppressible | partially by Oamb^UAS.K3, Scer\GAL4^GMR47A04

(Deady et al., 2017)

Scer\GAL4^GMR.PU, peb^GD1507 has axon | adult stage phenotype, suppressible | partially by jbug^UAS.L.Tag:HA/Scer\GAL4^GMR.PU

(Oliva et al., 2015)

Scer\GAL4^GMR.PU, peb^GD1507 has eye photoreceptor cell | adult stage phenotype, suppressible | partially by jbug^UAS.L.Tag:HA/Scer\GAL4^GMR.PU

(Oliva et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR44E10, peb^GD1507 has egg chamber | oogenesis stage S14 phenotype, non-suppressible by Oamb^UAS.K3, Scer\GAL4^GMR44E10

(Deady et al., 2017)

Scer\GAL4^GMR44E10, peb^GD1507 has oocyte associated follicular epithelium | oogenesis stage S14 phenotype, non-suppressible by Oamb^UAS.K3, Scer\GAL4^GMR44E10

(Deady et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of jbug^{Scer\UAS.L.T:Ivir\HA1} significantly partially suppresses axon targeting defects of R1-R6 photoreceptors in Scer\GAL4^GMR.PU>peb^GD1507 flies.

(Oliva et al., 2015)

Xenogenetic Interactions

Statement

Reference

add free text stating that the follicle rupture is even increased as compared to controls - Fig 8B - compare columns 1 and 3 - copy free text to Hsap\RREB1[UAS.EGFP].

(Deady et al., 2017)

Complementation and Rescue Data

Partially rescued by

peb^GD1507 is partially rescued by peb^EP55/Scer\GAL4^GMR44E10

(Deady et al., 2017)

peb^GD1507 is partially rescued by peb^EP55/Scer\GAL4^GMR47A04

(Deady et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)