Allele Dmel\pgant5^GD903

General Information
Symbol	Dmel\pgant5^GD903	Species	D. melanogaster
Name		FlyBase ID	FBal0210329
Feature type	allele	Associated gene	Dmel\pgant5

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..322
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD903} (Dickson et al., 2007.7.18, Mummery-Widmer et al., 2009, Tran et al., 2012)
Associated Sequence Features	dsRNA-GD903
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^c135 (Tran et al., 2012) lethal, with Scer\GAL4^c179 (Tran et al., 2012) lethal, with Scer\GAL4^αTub84B.PL (Tran et al., 2012) partially lethal - majority die, with Scer\GAL4^btl.PS (Tran et al., 2012) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	embryonic/larval cuprophilic cell, with Scer\GAL4^c135 (Tran et al., 2012) embryonic/larval gut, with Scer\GAL4^c135 (Tran et al., 2012)
Detailed Description
	Statement Reference Expression of pgant5[GD903] under the control of Scer\GAL4[αTub84B.PL] results in complete loss of viability. Expression of pgant5[GD903] in the digestive system under the control of Scer\GAL4[c135] results in complete lethality. Expression of pgant5[GD903] in the embryonic mesoderm under the control of Scer\GAL4[c179] results in complete lethality. Expression of pgant5[GD903] in the respiratory system under the control of Scer\GAL4[btl.PS] results in lethality. The digestive tracts of larvae expressing pgant5[GD903] under the control of Scer\GAL4[c135] show an unusually basic pH compared with controls. Wild-type copper cells display abundant O-glycoproteins throughout the apical and luminal region of copper cells. Larvae expressing pgant5[c03193] under the control of Scer\GAL4[c135] display a dramatic loss O-glycosylation normally found on copper cells and apical cytoskeletal abnormalities. Expression of pgant5[GD903] under the control of Scer\GAL4[Bx-MS1096] does not result in wing blistering. (Tran et al., 2012)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 903
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
VDRC	v2629 w¹¹¹⁸; P{GD903}v2629
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 903
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	pgant5^GD903
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Tran et al., 2012, J. Biol. Chem. 287(8): 5243--5252 Multiple Members of the UDP-GalNAc: Polypeptide N-Acetylgalactosaminyltransferase Family Are Essential for Viability in Drosophila. [FBrf0217507]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\pgant5GD903

Allele Dmel\pgant5^GD903