FlyBase Allele Report: Dmel\ple[GD1181]

General Information

Symbol

Dmel\ple^GD1181

Species

D. melanogaster

Name

FlyBase ID

FBal0210354

Feature type

allele

Associated gene

Dmel\ple

Associated Insertion(s)

Carried in Construct

P{GD1181}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1181}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1181

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates synucleinopathy

modeled by Hsap\SNCA^A30P.UAS

(Bayersdorfer et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal memory, with Scer\GAL4^ple.PF

(Lee et al., 2011)

decreased cell number | adult stage | drug conditional, with Scer\GAL4^ple.PF

(Bayersdorfer et al., 2010)

long lived, with Scer\GAL4^elav.PLu

(Bayersdorfer et al., 2010)

long lived | drug conditional, with Scer\GAL4^ple.PF

(Bayersdorfer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult cuticle, with Scer\GAL4^ple.PF

(Bayersdorfer et al., 2010)

dopaminergic neuron | drug conditional, with Scer\GAL4^ple.PF

(Bayersdorfer et al., 2010)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^en.PU

(Anh et al., 2011)

Detailed Description

Statement

Reference

Expression of ple^GD1181 under the control of Scer\GAL4^dimm-929 does not affect sensitivity to (S,Z,Z)-CH503 (a more potent stereoisomer of the gustatory sex pheromone CH503). As in wild type flies, courtship defects are seen in response to (S,Z,Z)-CH503.

(Shankar et al., 2015)

Expression of ple^GD1181 using Scer\GAL4^en.PU results in a paler posterior compartment. A held-out wing phenotype is not observed.

(Anh et al., 2011)

ple^GD1181-induced down regulation of ple in DPM neurons under the control of Scer\GAL4^VT64246 does not affect learning and 3 hour memory.

Expression of ple^GD1181 under the control of Scer\GAL4^ple.PF leads to impaired olfactory learning.

(Lee et al., 2011)

Flies expressing ple^GD1181 under the control of Scer\GAL4^ple.PF are viable and show a reduced cuticle pigmentation phenotype.

Rotenone treatment of flies expressing ple^GD1181 under the control of Scer\GAL4^ple.PF increases the mean life span by 20% with respect to Scer\GAL4^ple.PF controls, indicating rotenone toxicity is partially mediated by dopamine levels. Neuronal expression, under the control of Scer\GAL4^elav.PLu increases mean life span by 22%.

Flies expressing ple^GD1181 under the control of Scer\GAL4^ple.PF that are treated for 6 days with rotenone exhibit an approximate 20% loss of dopaminergic neurons, compared to 50% loss in control flies.

(Bayersdorfer et al., 2010)

Adults expressing ple^GD1181 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 100% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1181

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ple^GD1181, Scer\GAL4^ple.PF is a suppressor of abnormal neuroanatomy phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^ple.PF

(Bayersdorfer et al., 2010)

ple^GD1181, Scer\GAL4^ple.PF is a suppressor of abnormal locomotor behavior phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^ple.PF

(Bayersdorfer et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

ple^GD1181, Scer\GAL4^ple.PF is a suppressor of dopaminergic neuron phenotype of Hsap\SNCA^A30P.UAS, Scer\GAL4^ple.PF

(Bayersdorfer et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The number of dopaminergic neurons in Hsap\SNCA^{A30P.Scer\UAS} flies also expressing ple^GD1181 does not change from 3 to 35 days indicating a strong suppression effect by the ple^GD1181 line (when both are expressed under the control of Scer\GAL4^ple.PF).

Co-expression of ple^GD1181 with Hsap\SNCA^{A30P.Scer\UAS} (both under the control of Scer\GAL4^ple.PF) rescues the age-dependent decline in motor abilities seen upon Hsap\SNCA^{A30P.Scer\UAS} overexpression.

(Bayersdorfer et al., 2010)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v3308

w¹¹¹⁸; P{GD1181}v3308

Notes on Origin

Discoverer