FlyBase Allele Report: Dmel\polo[GD7563]

General Information

Symbol

Dmel\polo^GD7563

Species

D. melanogaster

Name

FlyBase ID

FBal0210372

Feature type

allele

Associated gene

Dmel\polo

Associated Insertion(s)

Carried in Construct

P{GD7563}

Also Known As

UAS-Polo RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7563}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7563

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Miro^UAS.Tag:MYC

(Lee et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centriole | adult stage | male, with Scer\GAL4^VP16.bam

(Varadarajan et al., 2016)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

spermatocyte, with Scer\GAL4^VP16.bam

(Varadarajan et al., 2016)

Detailed Description

Statement

Reference

Expression of polo^GD7563 under the control of Scer\GAL4^{bam.T:Hsim\VP16} blocks the centriole disengagement at meiosis I.

(Varadarajan et al., 2016)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in abnormally large neuroblasts greater than two times the normal cell diameter being seen in the larval brain.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an increase in the average size of neuroblasts in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing polo^GD7563 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing polo^GD7563 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7563

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

polo^GD7563/Scer\GAL4^VP16.bam is a suppressor of abnormal meiotic cell cycle | recessive phenotype of Myt1¹

(Varadarajan et al., 2016)

NOT Suppressor of

Statement

Reference

polo^GD7563/Scer\GAL4^VP16.bam is a non-suppressor of male sterile | recessive phenotype of Myt1¹

(Varadarajan et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

polo^GD7563/Scer\GAL4^VP16.bam is a suppressor | partially of spermatocyte phenotype of Scer\GAL4^VP16.bam, dbf^GD9284

(Sechi et al., 2019)

polo^GD7563/Scer\GAL4^VP16.bam is a suppressor of centriole | male | adult stage phenotype of Myt1¹

(Varadarajan et al., 2016)

NOT Suppressor of

Statement

Reference

polo^GD7563/Scer\GAL4^VP16.bam is a non-suppressor of fusome | male | adult stage phenotype of Myt1¹

(Varadarajan et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of polo^GD7563 under the control of Scer\GAL4^{bam.T:Hsim\VP16} partially suppresses the premature centriole disengagement observed in Myt1¹ homozygote mutant spermatocytes but has no effect on the fusome integrity defects and also does not rescue the male sterility.

(Varadarajan et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v20177