Expression of sbb^GD10734 under the control of Scer\GAL4^N630 (in combination with a heat shock to induce expression) leads to a decrease in the rate of male copulation success, and a wing vein phenotype wherein wing vein L5 fails to reach the wing margin.

Expression of sbb^GD10734 under the control of Scer\GAL4^l.Trh.PK in males leads to loss of serotonergic dsx-positive neurons in the abdominal ganglion, as compared to controls.

Expression of sbb^GD10734 under the control of Scer\GAL4^fru.P1.D or Scer\GAL4^fru-NP0021 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) leads to a significant decrease in the rate of male copulation success, and expression under the control of Scer\GAL4^fru.P1.D leads to a decrease in courtship index, however, expression of sbb^GD10734 under the control of Scer\GAL4^fru-NP0021 does not lead to any significant decrease in courtship index. Expression of sbb^GD10734 under the combinatorial control of Scer\GAL4^fru.P1.D, Scer\GAL80^{Scer\FRT.αTub84B} and Scer\FLP1^dsx does not lead to male courtship or copulation defects.

Expression of sbb^GD10734 under the control of Scer\GAL4^dsx.KI (with or without a Dicer-2 transgene to enhance RNAi efficiency) leads to a total lack of male copulation success and a significant decrease in courtship index, as compared to controls, but this phenotype is not seen upon inclusion of a Scer\GAL80^elav.PS transgene. Expression of sbb^GD10734 under the combinatorial control of Scer\GAL4^dsx.KI and Scer\GAL80^repo.PL, or of Scer\GAL4^dsx.KI, Scer\GAL80^{Scer\FRT.αTub84B} and Scer\FLP1^{o.FLP.oc.T:SV40\nls-largeT}, results in a complete lack of male copulation success, but no significant decrease in courtship index, as compared to controls. Expression of sbb^GD10734 under the combinatorial control of Scer\GAL4^dsx.KI, Scer\GAL80^αTub84B.PG and Scer\FLP1^fru, or of Scer\GAL4^dsx.KI, Scer\FLP1^{o.FLP.oc.T:SV40\nls-largeT} and Scer\GAL80^αTub84B.PG, does not lead to male courtship or copulation defects. Expression of sbb^GD10734 under the combinatorial control of Scer\GAL4^dsx.KI, Scer\GAL80^{IVS.WPRE.8xEcol\lexAop}, and Ecol\lexA^{fru.P1.T:Hsim\VP16}, or of Scer\GAL4^dsx.KI and Scer\GAL80^tsh-GAL80, results in a complete lack of male copulation success, and a decrease in courtship index, as compared to controls.

Expression of sbb^GD10734 under the combinatorial control of Scer\GAL80^αTub84B.PG and Scer\FLP1^dsx with Scer\GAL4^Poxn.14, Scer\GAL4^VGlut-OK371, Scer\GAL4^VGlut.PD, Scer\GAL4^ChAT.7.4, Scer\GAL4^ple.PF, Scer\GAL4^l.Trh.PK, Scer\GAL4^Gad1.3.098 or Scer\GAL4^Gad1.PS (but not with Scer\GAL4^ppk.PU or Scer\GAL4^Tdc2.PC) results in a complete lack of male copulation success. Expression of sbb^GD10734 under the combinatorial control of Scer\GAL80^αTub84B.PG and Scer\FLP1^dsx with either Scer\GAL4^Poxn.14 or Scer\GAL4^ppk.PU does not cause a significant decrease in courtship index, as compared to controls.

Adults expressing sbb^GD10734 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.