Allele Dmel\sds22^GD11788

General Information
Symbol	Dmel\sds22^GD11788	Species	D. melanogaster
Name		FlyBase ID	FBal0210539
Feature type	allele	Associated gene	Dmel\sds22

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..395
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD11788} (Dickson et al., 2007.7.18, Grusche et al., 2009, Jiang et al., 2011, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD11788
Cytology
Phenotypic Data
Phenotypic Class
	partially lethal - majority die \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	follicle cell \| somatic clone, with Scer\GAL4^Act.PU (Grusche et al., 2009) mesothoracic tergum, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) wing disc, with Scer\GAL4^ptc-559.1 (Jiang et al., 2011)
Detailed Description
	Statement Reference Cells expressing sds22[GD11788] under the control of Scer\GAL4[ptc-559.1] along the anterior-posterior compartment boundary of the wing disc are basally extruded and migrate away from the Scer\GAL4[ptc-559.1] expression domain into the posterior compartment (in contrast to controls, where the cells form a cohesive stripe in the Scer\GAL4[ptc-559.1] expression domain). This results in abnormal apical folding of the disc epithelium along the anterior-posterior compartment boundary. (Jiang et al., 2011) Cells in small clones in the follicular epithelium expressing sds22[GD11788] under the control of Scer\GAL4[Act] have a rounded appearance. (Grusche et al., 2009) Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4[pnr-MD237] expression domain. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 80-90% of the Scer\GAL4[pnr-MD237]-expressing area of the notum. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 11788
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Scer\GAL4^ptc-559.1, sds22^GD11788 has wing disc phenotype, suppressible \| partially by Scer\GAL4^ptc-559.1/puc^Scer\UAS.cMa (Jiang et al., 2011) Scer\GAL4^ptc-559.1, sds22^GD11788 has wing disc phenotype, suppressible \| partially by Scer\GAL4^ptc-559.1/Timp^Scer\UAS.cPa (Jiang et al., 2011) Scer\GAL4^ptc-559.1, sds22^GD11788 has wing disc phenotype, suppressible by Scer\GAL4^ptc-559.1/sqh^GD1695 (Jiang et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference The cell invasion and abnormal folding phenotypes seen in cells expressing sds22[GD11788] under the control of Scer\GAL4[ptc-559.1] along the anterior-posterior compartment boundary of the wing disc are suppressed if the cells are also co-expressing sqh[GD1695]. The cell invasion phenotype seen in cells expressing sds22[GD11788] under the control of Scer\GAL4[ptc-559.1] along the anterior-posterior compartment boundary of the wing disc is suppressed if the cells are also co-expressing puc[Scer\UAS.cMa], but the abnormal apical folding phenotype is not suppressed. The cell invasion phenotype seen in cells expressing sds22[GD11788] under the control of Scer\GAL4[ptc-559.1] along the anterior-posterior compartment boundary of the wing disc is largely suppressed if the cells are also co-expressing Timp[Scer\UAS.cPa], but the abnormal apical folding phenotype is not suppressed. (Jiang et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
VDRC	v42051 w¹¹¹⁸; P{GD11788}v42051
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 11788
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	sds22^GD11788
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Jiang et al., 2011, Oncogene 30(29): 3248--3260 Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling. [FBrf0214488] Grusche et al., 2009, BMC Dev. Biol. 9: 14 Sds22, a PP1 phosphatase regulatory subunit, regulates epithelial cell polarity and shape [Sds22 in epithelial morphology]. [FBrf0207451]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\sds22GD11788

Allele Dmel\sds22^GD11788