Allele Dmel\smt3^GD10521

General Information
Symbol	Dmel\smt3^GD10521	Species	D. melanogaster
Name		FlyBase ID	FBal0210631
Feature type	allele	Associated gene	Dmel\smt3

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :40..356 :1..39
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD10521} (Dickson et al., 2007.7.18, Nie et al., 2009, Smith et al., 2011, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD10521
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) mitotic cell cycle defective, with Scer\GAL4^Bx-MS1096 (Nie et al., 2009)
Phenotype Manifest In
	denticle belt, with Scer\GAL4^arm.PS (Smith et al., 2011) haltere, with Scer\GAL4^A9 (Smith et al., 2011) haltere \| somatic clone, with Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} (Smith et al., 2011) leg \| somatic clone, with Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} (Smith et al., 2011) macrochaeta, with Scer\GAL4^A9 (Smith et al., 2011) macrochaeta \| ectopic \| somatic clone, with Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} (Smith et al., 2011) wing, with Scer\GAL4^Bx-MS1096 (Nie et al., 2009) wing \| somatic clone, with Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} (Smith et al., 2011) wing pouch, with Scer\GAL4^Bx-MS1096 (Nie et al., 2009)
Detailed Description
	Statement Reference smt3[GD10521]-knockdown clones exhibit a range of defects including reduced wings and malformed legs. Haltere transformations range from the appearance of ectopic bristles to the significant outgrowth of wing tissue. Expression of smt3[GD10521] in the haltere under the control of Scer\GAL4[A9] results in multiple defects including ectopic bristle formation and increased size. Expression of smt3[GD10521] under the control of Scer\GAL4[arm.PS] results in embryos with missing or fused denticle belts. (Smith et al., 2011) Expression of smt3[GD10521] using Scer\GAL4[Bx-MS1096] results in a marked reduction of mitotic cells throughout the wing pouch of the wing disc and in the dorsal compartment in particular. Adult wings are greatly reduced in size and are misshapen. (Nie et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 10521
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
VDRC	v34113 w¹¹¹⁸; P{GD10521}v34113/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 10521
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	smt3^GD10521
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Smith et al., 2011, J. Biol. Chem. 286(13): 11391--11400 Small ubiquitin-like modifier (SUMO) conjugation impedes transcriptional silencing by the polycomb group repressor Sex Comb on Midleg. [FBrf0214411] Nie et al., 2009, PLoS ONE 4(6): e5905 Genetic and proteomic evidence for roles of Drosophila SUMO in cell cycle control, Ras signaling, and early pattern formation. [FBrf0208144]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\smt3GD10521

Allele Dmel\smt3^GD10521