FlyBase Allele Report: Dmel\sqh[GD1695]

General Information

Symbol

Dmel\sqh^GD1695

Species

D. melanogaster

Name

FlyBase ID

FBal0210676

Feature type

allele

Associated gene

Dmel\sqh

Associated Insertion(s)

Carried in Construct

P{GD1695}

Also Known As

UAS-sqh^RNAi, UAS-sqh RNAi, UAS-sqhRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1695}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1695

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antennal disc | larval stage, with Scer\GAL4^hth-GETDB

(Ku and Sun, 2017)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

Expression of sqh^GD1695 under the control of Scer\GAL4^hth-GETDB in the larval antennal disc leads to disorganization and a breakdown of the field boundary at the A1 fold, in contrast to controls, and mislocalized cells are maintained and integrated in the epithelial sheet.

(Ku and Sun, 2017)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an extreme increase in neuroblast size in the larval brain, without loss of neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in extreme defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing sqh^GD1695 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing sqh^GD1695 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1695

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

sqh^GD1695, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B is a suppressor of abnormal cell shape | conditional | third instar larval stage phenotype of BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B, sqh^GD1695 has abnormal cell shape | conditional | third instar larval stage phenotype

(Widmann and Dahmann, 2009)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/sqh^GD1695 is a suppressor of wing disc phenotype of Scer\GAL4^ptc-559.1, sds22^GD11788

(Jiang et al., 2011)

sqh^GD1695, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B is a suppressor of wing disc | conditional | third instar larval stage phenotype of BacA\p35^UAS.cHa, Dad^UAS.cTa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B

(Widmann and Dahmann, 2009)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, Scer\GAL80^ts.αTub84B, sqh^GD1695 has wing disc | conditional | third instar larval stage phenotype

(Widmann and Dahmann, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of sqh^GD1695 and BacA\p35^Scer\UAS.cHa in the dorsal compartment of the wing disc (under the temperature-regulated control of Scer\GAL4^ap-md544 by Scer\GAL80^ts.αTub84B) results in highly elongated apical-basal cell length in mutant cells compared with control cells.

The shorter cell length observed in wing disc tissue expressing Dad^Scer\UAS.cTa and BacA\p35^Scer\UAS.cHa (under the temperature-regulated control of Scer\GAL4^ap-md544 by Scer\GAL80^ts.αTub84B), is rescued by co-expression of sqh^GD1695.

(Widmann and Dahmann, 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v7916

w¹¹¹⁸; P{GD1695}v7916

v7917

w¹¹¹⁸; P{GD1695}v7917/TM3