FlyBase Allele Report: Dmel\wnd[GD13786]

General Information

Symbol

Dmel\wnd^GD13786

Species

D. melanogaster

Name

FlyBase ID

FBal0210894

Feature type

allele

Associated gene

Dmel\wnd

Associated Insertion(s)

Carried in Construct

P{GD13786}

Also Known As

UAS-wnd-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13786}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13786

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | third instar larval stage | conditional, with Scer\GAL4^tey-5053A

(Hao et al., 2016)

motor neuron | third instar larval stage | conditional, with Scer\GAL4^tey-5053A

(Hao et al., 2016)

NMJ bouton | larval stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Klinedinst et al., 2013)

Detailed Description

Statement

Reference

Expression of wnd^GD13786 under the control of Scer\GAL4^tey-5053A (in combination with a Dicer-2 transgene to enhance RNAi efficiency) leads to suppressed growth response of motoneuron axons after nerve crush injury in third instar larvae, as compared to wild type.

(Hao et al., 2016)

Expression of wnd^GD13786 under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486) results in an increase in maximum bouton diameter at the larval neuromuscular junction compared to controls.

(Klinedinst et al., 2013)

Adults expressing wnd^GD13786 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13786

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/wnd^GD13786, Scer\GAL80^ts.αTub84B is a suppressor | heat sensitive of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

wnd^GD13786, Scer\GAL4^tey-5053A is a suppressor of abnormal wound healing | third instar larval stage phenotype of Mmus\Prkaca^mC.UAS, Scer\GAL4^tey-5053A

(Hao et al., 2016)

wnd^GD13786, Scer\GAL4^tey-5053A is a suppressor of increased cell growth rate | third instar larval stage | conditional phenotype of Mmus\Prkaca^mC.UAS, Scer\GAL4^tey-5053A

(Hao et al., 2016)

Scer\GAL4^NP4132/wnd^GD13786 is a suppressor of abnormal memory phenotype of Scer\GAL4^NP4132, hiw^ΔRING.UAS

(Huang et al., 2012)

NOT Suppressor of

Statement

Reference

wnd^GD13786/Scer\GAL4^ptc.PU is a non-suppressor of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

Phenotype Manifest In

Suppressor of

Statement

Reference

wnd^GD13786, Scer\GAL4^tey-5053A is a suppressor of axon | third instar larval stage | conditional phenotype of Mmus\Prkaca^mC.UAS, Scer\GAL4^tey-5053A

(Hao et al., 2016)

wnd^GD13786, Scer\GAL4^tey-5053A is a suppressor of motor neuron | third instar larval stage | conditional phenotype of Mmus\Prkaca^mC.UAS, Scer\GAL4^tey-5053A

(Hao et al., 2016)

NOT Suppressor of

Statement

Reference

wnd^GD13786/Scer\GAL4^ptc.PU is a non-suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc.PU, lic^UAS.ORF

(Sun et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wnd^GD13786 suppresses the enhancement of aversive long term memory seen when hiw^{ΔRING.Scer\UAS} is expressed under the control of Scer\GAL4^NP4132. Learning is unaffected.

(Huang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Co-expression of wnd^GD13786 suppresses the enhanced growth response of motoneuron axons after nerve crush injury seen in larvae expressing Mmus\Pkaca^mC.Scer\UAS under the control of Scer\GAL4^tey-5053A.

(Hao et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v26910

w¹¹¹⁸; P{GD13786}v26910

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13786

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wnd^GD13786

(Wolfstetter et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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