FlyBase Allele Report: Dmel\wts[GD1563]

General Information

Symbol

Dmel\wts^GD1563

Species

D. melanogaster

Name

FlyBase ID

FBal0210898

Feature type

allele

Associated gene

Dmel\wts

Associated Insertion(s)

Carried in Construct

P{GD1563}

Also Known As

UAS-wts-RNAi, wts-RNAi, wts RNAi, UAS-RNAi-wts, UAS-wts RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1563}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1563

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Vigneswaran et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

increased cell number | pupal stage, with Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

lethal - all die before end of larval stage, with Scer\GAL4^en.PU

(Rauskolb et al., 2011)

lethal - all die before end of larval stage, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

some die during third instar larval stage, with Scer\GAL4^en.PU

(Rauskolb et al., 2011)

some die during third instar larval stage, with Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PFa, Scer\GAL4^ey.200.Exel

(Genevet et al., 2010)

visible, with Scer\GAL4^hh.PU

(Genevet et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PFa, Scer\GAL4^ey.200.Exel

(Genevet et al., 2010)

eye | pupal stage | somatic clone, with Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

interommatidial cell | pupal stage, with Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing blade posterior compartment, with Scer\GAL4^hh.PU

(Genevet et al., 2010)

Detailed Description

Statement

Reference

Pupal eye clones of cells expressing wts^GD1563 driven by Scer\GAL4^αTub84B.PL exhibit increased clonal area as well as overproliferation of interommatidial cells.

(Das Thakur et al., 2010)

Expression of wts^GD1563 under the control of Scer\GAL4^hh.PU results in overgrowth of the posterior wing compartment.

Expression of wts^GD1563 under the simultaneous control of both Scer\GAL4^GMR.PFa and Scer\GAL4^ey.200.Exel results in adults with increased eye size compared to controls. The retinas contain an excess of interommatidial cells.

(Genevet et al., 2010)

Adults expressing wts^GD1563 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1563

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^αTub84B.PL, wts^GD1563 has eye | pupal stage | somatic clone phenotype, non-enhanceable by Df(1)jub^I, Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PL, wts^GD1563 has eye | somatic clone | pupal stage phenotype, non-suppressible by Df(1)jub^I, Scer\GAL4^αTub84B.PL

(Das Thakur et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Homozygous mutant clones of Df(1)djub^I also expressing wts^GD1563 driven by Scer\GAL4^αTub84B.PL display phenotypes that resemble clones expressing wts^GD1563 alone.

(Das Thakur et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v9928

w¹¹¹⁸; P{GD1563}v9928

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1563

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

wts^GD1563

(Forest et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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