FlyBase Allele Report: Dmel\yki[GD11187]

General Information

Symbol

Dmel\yki^GD11187

Species

D. melanogaster

Name

FlyBase ID

FBal0210925

Feature type

allele

Associated gene

Dmel\yki

Associated Insertion(s)

Carried in Construct

P{GD11187}

Also Known As

UAS-yki-IR, UAS-yki-RNAi, UAS-yki^RNAi, UAS-YkiRNAi, UAS-yki RNAi, yki RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11187}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11187

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intestinal cancer

modeled by Ptp61F^HMS00421

(Liu et al., 2022)

ameliorates kidney disease

modeled by sdt^NIG.15342R

(Weide et al., 2017)

modeled by sdt^HMS01652

(Weide et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^nub.PU

(Hevia et al., 2017)

atrophy | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

decreased cell number | adult stage, with Scer\GAL4^nub.PU

(Venugopal et al., 2020)

decreased cell size | adult stage, with Scer\GAL4^nub.PU

(Venugopal et al., 2020)

decreased size | adult stage, with Scer\GAL4^nub.PU

(Venugopal et al., 2020)

flightless, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

increased size | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021, Schnorrer et al., 2010)

visible | adult stage, with Scer\GAL4^nub.PU

(Venugopal et al., 2020)

Phenotype Manifest In

indirect flight muscle cell | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

myotube | pupal stage, with Scer\GAL4^Mef2.PR

(Kaya-Çopur et al., 2021)

wing, with Scer\GAL4^nub.PU

(Venugopal et al., 2020, Hevia et al., 2017)

Detailed Description

Statement

Reference

The expression of yki^GD11187 under the control of Scer\GAL4^Mef2.PR leads to indirect flight muscle atrophy during pupal development (after 48h APF); fibers are longer than in controls, failing to compact at 32h APF.

(Kaya-Çopur et al., 2021)

The expression of yki^GD11187 under the control of Scer\GAL4^nub.PU leads to a significant decrease in wing size, as compared to controls.

(Hevia et al., 2017)

Expression of yki^GD11187 under the control of Scer\GAL4^Bx-MS1096 results in severly undergrown and malformed adult wings.

(Hu et al., 2016)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11187

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, yki^GD11187 has abnormal size | adult stage phenotype, enhanceable by Smox^NIG.2262R/Scer\GAL4^nub.PU

(Hevia et al., 2017)

Enhancer of

Statement

Reference

yki^GD11187/Scer\GAL4^nub.PU is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

yki^GD11187, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Ack^UAS.Tag:V5, Scer\GAL4^GMR.PU

(Hu et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/yki^GD11187 is a suppressor of increased size | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, Toll-7^{C993Y.UAS.Tag:FLAG}

(Ding et al., 2022)

yki^GD11187/Scer\GAL4^nub.PU is a suppressor | partially of abnormal size | adult stage phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

yki^GD11187, Scer\GAL4^Bx-MS1096 is a suppressor of visible | adult stage phenotype of Ack^UAS.Tag:V5, Scer\GAL4^Bx-MS1096

(Hu et al., 2016)

Scer\GAL4^NP0001/yki^GD11187 is a suppressor of decreased body size phenotype of Pez²/Pez¹

(Poernbacher et al., 2012)

Scer\GAL4^NP0001/yki^GD11187 is a suppressor of increased occurrence of cell division | adult stage phenotype of Pez²/Pez¹

(Poernbacher et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, yki^GD11187 has wing phenotype, enhanceable by Smox^NIG.2262R/Scer\GAL4^nub.PU

(Hevia et al., 2017)

Enhancer of

Statement

Reference

yki^GD11187/Scer\GAL4^nub.PU is an enhancer of wing phenotype of Scer\GAL4^nub.PU, Smox^NIG.2262R

(Hevia et al., 2017)

yki^GD11187, Scer\GAL4^GMR.PU is an enhancer of eye | adult stage phenotype of Ack^UAS.Tag:V5, Scer\GAL4^GMR.PU

(Hu et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^ptc-559.1/yki^GD11187 is a suppressor of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, Toll-7^{C993Y.UAS.Tag:FLAG}

(Ding et al., 2022)

yki^GD11187/Scer\GAL4^nub.PU is a suppressor | partially of wing phenotype of Scer\GAL4^nub.PU, Smox^SDVD.UAS

(Hevia et al., 2017)

yki^GD11187, Scer\GAL4^Bx-MS1096 is a suppressor of wing blade phenotype of Ack^UAS.Tag:V5, Scer\GAL4^Bx-MS1096

(Hu et al., 2016)

Scer\GAL4^NP0001/yki^GD11187 is a suppressor of adult midgut epithelium phenotype of Pez²/Pez¹

(Poernbacher et al., 2012)

Scer\GAL4^NP0001, yki^GD11187, Scer\GAL80^ts.αTub84B is a suppressor | heat sensitive | partially of adult midgut epithelium phenotype of Pez²/Pez¹

(Poernbacher et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased wing size induced by the expression of either yki^GD11187 or Smox^NIG.2262R under the control of Scer\GAL4^nub.PU (together with Dicer-2, for efficient RNAi) becomes more severe upon the co-expression of both.

The co-expression of yki^GD11187 (together with Dicer-2, for efficient RNAi) partially suppresses the severely increased wing size induced by the expression of Smox^{SDVD.Scer\UAS} under the control of Scer\GAL4^nub.PU.

(Hevia et al., 2017)

The increased adult wing size characteristic for flies expressing Ack^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^Bx-MS1096 is completely suppressed by coexpression of yki^GD11187 : the resulting wings are severely undergrown and malformed compared to wild-type and their size is not significantly different from when yki^GD11187 is expressed on its own.

(Hu et al., 2016)

Expression of yki^GD11187 under the control of Scer\GAL4^{Myo31DF-NP0001} suppresses the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults and restores the body size of Pez¹/Pez² adults to normal.

Expression of yki^GD11187 under the control of Scer\GAL4^{Myo31DF-NP0001} during the adult stage only (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit the stage of expression) partially suppresses the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

Expression of yki^GD11187 under the control of Scer\GAL4^{Myo31DF-NP0001} during the adult stage only (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit the stage of expression) has no clear effect on the increased number of mitotic cells seen in the midgut of Pez¹/Pez² adults.

(Poernbacher et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v40497

w¹¹¹⁸; P{GD11187}v40497/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11187

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

yki^GD11187

Name Synonyms

Secondary FlyBase IDs

References (25)

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