Allele Dmel\Or43a^{Scer\UAS.T:Avic\GFP-YFP(2)}

General Information
Symbol	Dmel\Or43a^{Scer\UAS.T:Avic\GFP-YFP(2)}	Species	D. melanogaster
Name		FlyBase ID	FBal0210983
Feature type	allele	Associated gene	Dmel\Or43a

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Benton et al., 2006) in vitro construct - regulatory fusion (Benton et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Or43a, which is tagged with an C-terminal fragment of T:Avic\GFP-YFP. (Benton et al., 2006)
Carried in construct	P{UAS-Or43a.YFP(2)} (Benton et al., 2006)
Tagged with	T:Avic\GFP^YFP (Benton et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	This construct is designed for a "protein-fragment complementation assay (PCA)"; the T:Avic\GFP-YFP C-terminal fragment is complementary to an N-terminal T:Avic\GFP-YFP fragment which is carried on separate constructs. Binding of proteins tagged with either of the two T:Avic\GFP-YFP fragments brings them in close enough proximity to fold into an active form and fluoresce. (Benton et al., 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Or43a^{Scer\UAS.T:Avic\GFP-YFP(2)}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Benton et al., 2006, PLoS Biol. 4(2): e20 Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo. [FBrf0192657]

Allele Dmel\Or43aScer\UAS.T:Avic\GFP-YFP(2)

Allele Dmel\Or43a^{Scer\UAS.T:Avic\GFP-YFP(2)}