Avic\GFPStat92E.10X reporter activity is highly asymmetric within the anterior of the stage 14 large intestine. Cells on the inside of the hindgut curve show higher pathway activity than cells at comparable distances from the Upd-expressing large intestine on the outside of the curve. This difference in reporter activity is significant and not a consequence of increased cell density. Asymmetry in reporter activity is mirrored by expression of the endogenous JAK/STAT pathway target gene Socs36E.
In second instar larvae, reporter expression is observed throughout the disc proper. By early third instar, expression is repressed in the distal pouch and proximal notum,conincident with nub and eyg expression in these regions.
This GFP-reporter of JAK-STAT signaling is specifically expressed in the cells at the junction of the foregut and the midgut in third instar larvae.